Wilkie T M, Palmiter R D
Mol Cell Biol. 1987 May;7(5):1646-55. doi: 10.1128/mcb.7.5.1646-1655.1987.
Males in the transgenic mouse pedigree MyK-103, although fertile, do not transmit the integrant to offspring. The integrant is on chromosome 6 near the T-cell receptor beta-chain locus. It contains four fragments of the plasmid pMK (a metallothionein-thymidine kinase fusion gene) and a 532-base-pair fragment of displaced mouse DNA originating from a previously uncharacterized repetitive DNA family. The integration complex is flanked on either side by a 5-kilobase duplication of mouse DNA normally found in a single copy at this locus. Sequence analysis of the six novel junctions and their donor sequences shows that plasmid-plasmid junctions occurred at patches of limited homology, whereas chromosome-plasmid junctions were nonhomologous.
转基因小鼠谱系MyK - 103中的雄性小鼠虽然可育，但不会将整合体传递给后代。该整合体位于6号染色体上靠近T细胞受体β链基因座的位置。它包含质粒pMK的四个片段（一个金属硫蛋白 - 胸苷激酶融合基因）以及一段532个碱基对的小鼠DNA片段，该片段源自一个先前未被鉴定的重复DNA家族。整合复合体两侧各有一段5千碱基对的小鼠DNA重复序列，该序列在此基因座通常以单拷贝形式存在。对六个新的连接点及其供体序列的序列分析表明，质粒 - 质粒连接点出现在有限同源性的区域，而染色体 - 质粒连接点则是非同源的。
