Beijing Synchrotron Radiation Facility, Institute of High Energy Physics , Chinese Academy of Sciences , Beijing 100049 , China.
CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety , National Center for NanoScience and Technology , Beijing 100190 , China.
Langmuir. 2018 Oct 23;34(42):12583-12589. doi: 10.1021/acs.langmuir.8b01700. Epub 2018 Oct 9.
This report clarifies the interaction of surface charge-modified gold nanoparticles (AuNPs) with phospholipid membranes, which is helpful to understand the antibacterial mechanism of positive charge-modified AuNPs to Gram-negative bacteria. Although the simulated bacterial cell membranes as a whole are negatively charged, the local electrostatic repulsive interaction between the positive charge-coated AuNPs and the small-sized flexible cationic head group of dioleyl phosphatidylethanolamine molecules induces the phase transformation of the simulated bacterial cell membranes from a lamellar to an inverted hexagonal phase. Transmembrane pores with a diameter of about 3.0 nm in the inverted hexagonal structure would result in the destruction of cell membrane function. Such an interaction of positive charge-modified AuNPs with the membrane mimics provides a promising route to develop new antibacterial agents by modifying positive charges on the surface of nanoparticles.
本报告阐明了表面电荷修饰的金纳米粒子（AuNPs）与磷脂膜的相互作用，这有助于理解正电荷修饰的 AuNPs 对革兰氏阴性菌的抗菌机制。尽管模拟的细菌细胞膜整体带负电荷，但带正电荷的 AuNPs 与二油酰基磷脂酰乙醇胺分子的小尺寸柔性阳离子头基团之间的局部静电排斥相互作用诱导模拟细菌细胞膜从层状向反相六方相转变。反相六方结构中直径约为 3.0nm 的跨膜孔会导致细胞膜功能的破坏。这种带正电荷的 AuNPs 与膜模拟物的相互作用为通过修饰纳米粒子表面的正电荷来开发新型抗菌剂提供了有前途的途径。
