Hertz G Z, Young M R, Mertz J E
J Virol. 1987 Jul;61(7):2322-5. doi: 10.1128/JVI.61.7.2322-2325.1987.
The origin-promoter region of simian virus 40 contains a 17-base-pair sequence composed exclusively of adenine (A) and thymine (T). We constructed a linker replacement mutant in which this stretch of A's and T's was reduced to 11 base pairs. While not affecting the level of early gene transcription, this mutation reduced the accumulation of viral DNA in COS cells at least 10(4) fold. In addition, a restriction fragment containing the wild-type A + T-rich region migrated in nondenaturing polyacrylamide gels with an anomalous mobility characteristic of bent DNA; however, the corresponding fragment from the mutant migrated less anomalously. Therefore, bending of the DNA in this region may play a role in some step in viral DNA replication.
猴病毒40的起始启动子区域包含一段仅由腺嘌呤（A）和胸腺嘧啶（T）组成的17个碱基对的序列。我们构建了一个连接子替换突变体，其中这段A和T序列被减少到11个碱基对。该突变虽不影响早期基因转录水平，但使病毒DNA在COS细胞中的积累至少降低了10⁴倍。此外，一个包含野生型富含A + T区域的限制性片段在非变性聚丙烯酰胺凝胶中迁移时具有弯曲DNA的异常迁移特性；然而，来自突变体的相应片段迁移时异常程度较小。因此，该区域DNA的弯曲可能在病毒DNA复制的某些步骤中起作用。
