用于研究肠内分泌细胞的模型和工具。
Models and Tools for Studying Enteroendocrine Cells.
机构信息
Metabolic Research Laboratories, University of Cambridge, Cambridge, United Kingdom.
出版信息
Endocrinology. 2018 Dec 1;159(12):3874-3884. doi: 10.1210/en.2018-00672.
Abstract
Gut hormones produced by gastrointestinal enteroendocrine cells modulate key physiological processes including glucose homeostasis and food intake, making them potential therapeutic candidates to treat obesity and diabetes. Understanding the function of enteroendocrine cells and the molecular mechanisms driving hormone production is a key step toward mobilizing endogenous hormone reserves in the gut as a therapeutic strategy. In this review, we will discuss the variety of ex vivo and in vitro model systems driving this research and their contributions to our current understanding of nutrient-sensing mechanisms in enteroendocrine cells.
摘要
胃肠道内分泌细胞产生的肠激素调节包括葡萄糖稳态和摄食在内的关键生理过程，使它们成为治疗肥胖症和糖尿病的潜在治疗候选物。了解内分泌细胞的功能和驱动激素产生的分子机制是将肠道内源性激素储备作为治疗策略的关键步骤。在这篇综述中，我们将讨论推动这一研究的各种离体和体外模型系统及其对我们当前理解肠内分泌细胞中营养感应机制的贡献。
相似文献
1
Models and Tools for Studying Enteroendocrine Cells.用于研究肠内分泌细胞的模型和工具。
Endocrinology. 2018 Dec 1;159(12):3874-3884. doi: 10.1210/en.2018-00672.
2
Enteroendocrine Cells: Chemosensors in the Intestinal Epithelium.肠内分泌细胞：肠道上皮中的化学传感器
Annu Rev Physiol. 2016;78:277-99. doi: 10.1146/annurev-physiol-021115-105439. Epub 2015 Oct 6.
3
Gut chemosensing mechanisms.肠道化学传感机制。
J Clin Invest. 2015 Mar 2;125(3):908-17. doi: 10.1172/JCI76309. Epub 2015 Feb 9.
4
Enteroendocrine Systems That Sense Nutrients in the Gut and Control the Body.肠道内感受肠道内营养素并调节机体的内感受器系统。
J Nutr Sci Vitaminol (Tokyo). 2022;68(Supplement):S5-S7. doi: 10.3177/jnsv.68.S5.
5
Diet: friend or foe of enteroendocrine cells--how it interacts with enteroendocrine cells.饮食：肠内分泌细胞的朋友还是敌人——它与肠内分泌细胞如何相互作用。
Adv Nutr. 2012 Jan;3(1):8-20. doi: 10.3945/an.111.000976. Epub 2012 Jan 5.
6
Gut motility and enteroendocrine secretion.肠道动力与肠内分泌分泌。
Curr Opin Pharmacol. 2013 Dec;13(6):928-34. doi: 10.1016/j.coph.2013.09.002. Epub 2013 Sep 20.
7
The Changing Concept of Gut Endocrinology.肠道内分泌学概念的演变
Endocr Dev. 2017;32:8-19. doi: 10.1159/000475728. Epub 2017 Aug 15.
8
Enteroendocrine cells: a review of their role in brain-gut communication.肠内分泌细胞：它们在脑-肠通讯中作用的综述
Neurogastroenterol Motil. 2016 May;28(5):620-30. doi: 10.1111/nmo.12754. Epub 2015 Dec 21.
9
Gastrointestinal targets to modulate satiety and food intake.调节饱腹感和食物摄入的胃肠道靶点。
Obes Rev. 2011 Jun;12(6):470-7. doi: 10.1111/j.1467-789X.2010.00788.x. Epub 2010 Sep 6.
10
Gastrointestinal hormones and the gut connectome.胃肠激素与肠道连接组
Curr Opin Endocrinol Diabetes Obes. 2017 Feb;24(1):9-14. doi: 10.1097/MED.0000000000000299.
引用本文的文献
1
Enteroendocrine cells: the gatekeepers of microbiome-gut-brain communication.肠内分泌细胞：微生物群-肠道-大脑通讯的守门人。
NPJ Biofilms Microbiomes. 2025 Sep 1;11(1):179. doi: 10.1038/s41522-025-00810-x.
2
Gut Microbial Metabolites of Tryptophan Augment Enteroendocrine Cell Differentiation in Human Colonic Organoids: Therapeutic Potential for Dysregulated GLP1 Secretion in Obesity.色氨酸的肠道微生物代谢产物促进人结肠类器官中肠内分泌细胞分化：肥胖中胰高血糖素样肽-1分泌失调的治疗潜力
Int J Mol Sci. 2025 Jul 23;26(15):7080. doi: 10.3390/ijms26157080.
3
Mechanics of serotonin-producing human entero-endocrine cells.产生血清素的人类肠内分泌细胞的力学特性
Mechanobiol Med. 2024 Feb 8;2(2):100044. doi: 10.1016/j.mbm.2024.100044. eCollection 2024 Jun.
4
Insights into GLP-1 and insulin secretion mechanisms in pasireotide-induced hyperglycemia highlight effectiveness of Gs-targeting diabetes treatment.对帕西瑞肽诱导高血糖症中GLP-1和胰岛素分泌机制的深入了解凸显了靶向Gs的糖尿病治疗的有效性。
Sci Rep. 2025 Mar 19;15(1):9494. doi: 10.1038/s41598-025-90896-2.
5
Protein absorption in the zebrafish gut is regulated by interactions between lysosome rich enterocytes and the microbiome.斑马鱼肠道中的蛋白质吸收受富含溶酶体的肠上皮细胞与微生物群之间相互作用的调节。
Elife. 2025 Mar 13;13:RP100611. doi: 10.7554/eLife.100611.
6
Understanding the release mechanisms and secretion patterns for glucagon-like peptide-1 using the isolated perfused intestine as a model.以离体灌注肠段为模型，了解胰高血糖素样肽-1的释放机制和分泌模式。
Biochem Soc Trans. 2025 Jan 31;53(1):BST20241062. doi: 10.1042/BST20241062.
7
Crosstalk Within the Intestinal Epithelium: Aspects of Intestinal Absorption, Homeostasis, and Immunity.肠上皮内的串扰：肠道吸收、稳态及免疫的相关方面
Biomedicines. 2024 Dec 5;12(12):2771. doi: 10.3390/biomedicines12122771.
8
Protein absorption in the zebrafish gut is regulated by interactions between lysosome rich enterocytes and the microbiome.斑马鱼肠道中的蛋白质吸收受富含溶酶体的肠上皮细胞与微生物群之间相互作用的调节。
bioRxiv. 2024 Dec 3:2024.06.07.597998. doi: 10.1101/2024.06.07.597998.
9
Single Cell Analysis of Human Colonoids Exposed to Uranium-Bearing Dust.人类类器官暴露于含铀粉尘后的单细胞分析
Environ Health Perspect. 2024 May;132(5):57006. doi: 10.1289/EHP13855. Epub 2024 May 21.
10
A human stomach cell type transcriptome atlas.人类胃细胞类型转录组图谱。
BMC Biol. 2024 Feb 14;22(1):36. doi: 10.1186/s12915-024-01812-5.
本文引用的文献
1
Co-storage and release of insulin-like peptide-5, glucagon-like peptide-1 and peptideYY from murine and human colonic enteroendocrine cells.肠内分泌细胞共储存和释放肠促胰岛素样肽 5、胰高血糖素样肽 1 和肽 YY（来自鼠和人结肠）。
Mol Metab. 2018 Oct;16:65-75. doi: 10.1016/j.molmet.2018.07.011. Epub 2018 Jul 30.
2
Bile acids are important direct and indirect regulators of the secretion of appetite- and metabolism-regulating hormones from the gut and pancreas.胆汁酸是调节肠道和胰腺分泌食欲和代谢调节激素的重要直接和间接调节剂。
Mol Metab. 2018 May;11:84-95. doi: 10.1016/j.molmet.2018.03.007. Epub 2018 Mar 17.
3
Enterochromaffin 5-HT cells - A major target for GLP-1 and gut microbial metabolites.肠嗜铬细胞 5-HT - GLP-1 和肠道微生物代谢物的主要靶点。
Mol Metab. 2018 May;11:70-83. doi: 10.1016/j.molmet.2018.03.004. Epub 2018 Mar 10.
4
Gastrectomy with Roux-en-Y reconstruction as a lean model of bariatric surgery.胃切除术联合 Roux-en-Y 重建术作为一种减重手术的瘦素模型。
Surg Obes Relat Dis. 2018 May;14(5):562-568. doi: 10.1016/j.soard.2018.01.039. Epub 2018 Feb 3.
5
Bidirectional GPR119 Agonism Requires Peptide YY and Glucose for Activity in Mouse and Human Colon Mucosa.双向 GPR119 激动剂在人和鼠结肠黏膜中的活性需要肽 YY 和葡萄糖。
Endocrinology. 2018 Apr 1;159(4):1704-1717. doi: 10.1210/en.2017-03172.
6
Mechanistic insights into the detection of free fatty and bile acids by ileal glucagon-like peptide-1 secreting cells.肠促胰素 GLP-1 分泌细胞检测游离脂肪酸和胆汁酸的机制研究。
Mol Metab. 2018 Jan;7:90-101. doi: 10.1016/j.molmet.2017.11.005. Epub 2017 Nov 11.
7
A single-cell survey of the small intestinal epithelium.小肠上皮的单细胞调查。
Nature. 2017 Nov 16;551(7680):333-339. doi: 10.1038/nature24489. Epub 2017 Nov 8.
8
Sodium channel Na1.3 is important for enterochromaffin cell excitability and serotonin release.钠离子通道 Na1.3 对肠嗜铬细胞的兴奋性和 5-羟色胺释放很重要。
Sci Rep. 2017 Nov 15;7(1):15650. doi: 10.1038/s41598-017-15834-3.
9
Single-cell RNA-sequencing reveals a distinct population of proglucagon-expressing cells specific to the mouse upper small intestine.单细胞 RNA 测序揭示了一种独特的胰高血糖素前体细胞群，其特异性存在于小鼠的上小肠中。
Mol Metab. 2017 Oct;6(10):1296-1303. doi: 10.1016/j.molmet.2017.07.014. Epub 2017 Aug 1.
10
Liquid chromatography/mass spectrometry based detection and semi-quantitative analysis of INSL5 in human and murine tissues.基于液相色谱/质谱联用技术对人和小鼠组织中胰岛素样肽5（INSL5）的检测及半定量分析
Rapid Commun Mass Spectrom. 2017 Dec 15;31(23):1963-1973. doi: 10.1002/rcm.7978.
Zotero 插件