Keebaugh Erin S, Yamada Ryuichi, Obadia Benjamin, Ludington William B, Ja William W
Department of Neuroscience, The Scripps Research Institute, Jupiter, FL 33458, USA; Center on Aging, The Scripps Research Institute, Jupiter, FL 33458, USA.
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
iScience. 2018 Jun 29;4:247-259. doi: 10.1016/j.isci.2018.06.004. Epub 2018 Jun 8.
In Drosophila, microbial association can promote development or extend life. We tested the impact of microbial association during malnutrition and show that microbial quantity is a predictor of fly longevity. Although all tested microbes, when abundantly provided, can rescue lifespan on low-protein diet, the effect of a single inoculation seems linked to the ability of that microbial strain to thrive under experimental conditions. Microbes, dead or alive, phenocopy dietary protein, and the calculated dependence on microbial protein content is similar to the protein requirements determined from fly feeding studies, suggesting that microbes enhance host protein nutrition by serving as protein-rich food. Microbes that enhance larval growth are also associated with the ability to better thrive on fly culture medium. Our results suggest an unanticipated range of microbial species that promote fly development and longevity and highlight microbial quantity as an important determinant of effects on physiology and lifespan during undernutrition.
在果蝇中，与微生物的关联可以促进发育或延长寿命。我们测试了营养不良期间微生物关联的影响，并表明微生物数量是果蝇寿命的一个预测指标。尽管所有测试的微生物在大量提供时都能挽救低蛋白饮食条件下的寿命，但单次接种的效果似乎与该微生物菌株在实验条件下的生长能力有关。活的或死的微生物都能模拟膳食蛋白质的作用，计算得出的对微生物蛋白质含量的依赖性与从果蝇喂养研究中确定的蛋白质需求相似，这表明微生物通过作为富含蛋白质的食物来增强宿主的蛋白质营养。促进幼虫生长的微生物也与在果蝇培养基上更好生长的能力有关。我们的结果表明，有一系列意想不到的微生物物种能够促进果蝇的发育和延长寿命，并突出了微生物数量是营养不良期间对生理和寿命影响的一个重要决定因素。
