嗜热栖热放线菌内切葡聚糖酶D编码基因celD的核苷酸序列
Nucleotide sequence of the cellulase gene celD encoding endoglucanase D of Clostridium thermocellum.
作者信息
Joliff G, Béguin P, Aubert J P
出版信息
Nucleic Acids Res. 1986 Nov 11;14(21):8605-13. doi: 10.1093/nar/14.21.8605.
Abstract
The nucleotide sequence of the celD gene, encoding the previously crystallized endoglucanase D of Clostridium thermocellum, is reported. The enzyme shares a conserved, reiterated domain with the COOH-terminal end of endoglucanases A and B from the same organism. The overexpression in Escherichia coli of celD subcloned in pUC8 appears to result from a translational fusion of the NH2-terminal end of the endoglucanase with the NH2-terminal end of beta-galactosidase.
摘要
报道了编码热纤梭菌先前已结晶的内切葡聚糖酶D的celD基因的核苷酸序列。该酶与来自同一生物体的内切葡聚糖酶A和B的COOH末端共享一个保守的重复结构域。克隆于pUC8的celD在大肠杆菌中的过表达似乎是由于内切葡聚糖酶的NH2末端与β-半乳糖苷酶的NH2末端发生了翻译融合。
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