Department of Chemistry, University of Bath, Bath, BA2 7AY, UK.
Chem Commun (Camb). 2018 Oct 4;54(80):11336-11339. doi: 10.1039/c8cc06917d.
An ESIPT-based 'AND' logic fluorescence probe (GSH-ABAH) was developed for the simultaneous detection of ONOO- and biological thiols. GSH-ABAH was shown to have good cell permeability and with the addition of just SIN-1 (ONOO- donor) or GSH, no fluorescence response was observed in live cells. However, in the presence of both analytes GSH-ABAH could be used to image exogenous ONOO- 'AND' GSH added to RAW264.7 cells.
基于 ESIPT 的“与”逻辑荧光探针（GSH-ABAH）被开发用于同时检测 ONOO-和生物硫醇。结果表明，GSH-ABAH 具有良好的细胞通透性，并且仅加入 SIN-1（ONOO-供体）或 GSH 时，在活细胞中未观察到荧光响应。然而，在同时存在两种分析物的情况下，GSH-ABAH 可用于对 RAW264.7 细胞中外源性 ONOO-和添加的 GSH 进行成像。
