Dennis Kristine K, Carter Brian D, Gapstur Susan M, Stevens Victoria L
Nutrition and Health Sciences, Laney Graduate School, Emory University 615 Michael Street, Atlanta, GA 30322, USA.
Behavioral and Epidemiology Research Group, American Cancer Society, 250 Williams Street, NW, Atlanta, GA 30303, USA.
Metabolites. 2018 Sep 21;8(4):55. doi: 10.3390/metabo8040055.
Over-the-counter analgesic use is common and is typically assessed through self-report; therefore, it is subject to misclassification. Detection of drug metabolites in biofluids offers a viable tool for validating self-reported analgesic use. Thus, the aim of this study was to determine the utility of a metabolomics approach for the validation of acetaminophen and ibuprofen use in blood samples. Untargeted mass spectrometry-based metabolomics analysis was conducted in serum samples from 1547 women and plasma samples from 556 men. The presence of two metabolites each for acetaminophen and ibuprofen at levels at or above a defined cutoff value was used to determine concordance with self-reported use. For acetaminophen use based on the presence of both acetaminophen and acetamidophenylglucuronide, concordance was 98.5⁻100% among individuals reporting use today, and 79.8⁻91.4% for those reporting never or rare use. Ibuprofen use based on the presence of both carboxyibuprofen and hydroxyibuprofen resulted in concordance of 51.3⁻52.5% for individuals reporting use today and 99.4⁻100% for those reporting never or rare use. Our findings suggest that an untargeted metabolomics approach in blood samples may be useful for validating self-reported acetaminophen use. However, this approach appears unlikely to be suitable for validating ibuprofen use.
非处方镇痛药的使用很常见，通常通过自我报告进行评估；因此，存在分类错误的可能性。检测生物流体中的药物代谢物为验证自我报告的镇痛药使用情况提供了一种可行的工具。因此，本研究的目的是确定代谢组学方法在验证血液样本中对乙酰氨基酚和布洛芬使用情况的效用。对1547名女性的血清样本和556名男性的血浆样本进行了基于非靶向质谱的代谢组学分析。根据对乙酰氨基酚和布洛芬各自两种代谢物的水平是否达到或高于定义的临界值来确定与自我报告使用情况的一致性。对于基于对乙酰氨基酚和对乙酰氨基酚葡糖醛酸苷的存在情况来判断对乙酰氨基酚的使用，在报告当天使用的个体中一致性为98.5%-100%，而在报告从不或很少使用的个体中为79.8%-91.4%。基于羧基布洛芬和羟基布洛芬的存在情况来判断布洛芬的使用，在报告当天使用的个体中一致性为51.3%-52.5%，而在报告从不或很少使用的个体中为99.4%-100%。我们的研究结果表明，血液样本中的非靶向代谢组学方法可能有助于验证自我报告的对乙酰氨基酚使用情况。然而，这种方法似乎不太适合验证布洛芬的使用情况。
