Wang Xin-You, Liu Ying
Qinghai Academy of Animal and Veterinary Sciences, Qinghai Provincial Key Laboratory of Adaptive Management on Alpine Grassland, Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau, Qinghai University, Xining, China.
Mitochondrial DNA B Resour. 2023 Aug 2;8(8):819-822. doi: 10.1080/23802359.2023.2238358. eCollection 2023.
Willd. is an important medicinal plant and can also be used as a forage grass. To better understand the diversity and phylogeny between and other species, we sequenced and annotated the complete chloroplast genome of by using the Illumina Hiseq 2500 platform. This complete chloroplast genome is 152,473 bp long and the GC content is 37.3% presented a negative AT-skew (-0.002) and a positive GC-skew (0.003). The genome contains a large single-copy region (LSC) of 84,235 bp, a small single-copy region (SSC) of 18,212 bp, which separated by a pair of inverted repeat regions (IRA and IRB) of 25,013 bp. Moreover, 129 genes were found in the chloroplast of , including 85 protein-coding genes (PCGs), 36 transfer RNA genes (tRNAs), 8 ribosomal RNA unit genes (rRNAs). Phylogenetic analysis showed that was more closely related to and var. uchiyamae. This study lays the foundation for further studies on the evolution and phylogeny of .
Willd.是一种重要的药用植物，也可作为饲料草。为了更好地了解Willd.与其他物种之间的多样性和系统发育关系，我们利用Illumina Hiseq 2500平台对Willd.的完整叶绿体基因组进行了测序和注释。这个完整的叶绿体基因组长度为152,473 bp，GC含量为37.3%，呈现出负的AT偏斜（-0.002）和正的GC偏斜（0.003）。基因组包含一个84,235 bp的大单拷贝区域（LSC）、一个18,212 bp的小单拷贝区域（SSC），它们被一对25,013 bp的反向重复区域（IRA和IRB）隔开。此外，在Willd.的叶绿体中发现了129个基因，包括85个蛋白质编码基因（PCGs）、36个转运RNA基因（tRNAs）、8个核糖体RNA单位基因（rRNAs）。系统发育分析表明，Willd.与[物种名]和[物种名]变种uchiyamae关系更密切。本研究为进一步研究Willd.的进化和系统发育奠定了基础。
