Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Seeland OT Gatersleben, Germany.
Department of Bioinformatics, Martin-Luther-University, 06120, Halle, Germany.
New Phytol. 2019 Jan;221(1):261-278. doi: 10.1111/nph.15439. Epub 2018 Sep 25.
Plant-specific EFFECTORS OF TRANSCRIPTION (ET) are characterised by a variable number of highly conserved ET repeats, which are involved in zinc and DNA binding. In addition, ETs share a GIY-YIG domain, involved in DNA nicking activity. It was hypothesised that ETs might act as epigenetic regulators. Here, methylome, transcriptome and phenotypic analyses were performed to investigate the role of ET factors and their involvement in DNA methylation in Arabidopsis thaliana. Comparative DNA methylation and transcriptome analyses in flowers and seedlings of et mutants revealed ET-specific differentially expressed genes and mostly independently characteristic, ET-specific differentially methylated regions. Loss of ET function results in pleiotropic developmental defects. The accumulation of cyclobutane pyrimidine dimers after ultraviolet stress in et mutants suggests an ET function in DNA repair.
植物特异性转录因子（ET）的特征是具有数量可变的高度保守的 ET 重复序列，这些重复序列参与锌和 DNA 的结合。此外，ET 还具有一个 GIY-YIG 结构域，该结构域参与 DNA 缺口活性。有人假设 ET 可能作为表观遗传调节剂发挥作用。在这里，进行了甲基组、转录组和表型分析，以研究 ET 因子在拟南芥中的作用及其在 DNA 甲基化中的参与。在 et 突变体的花和幼苗中的比较 DNA 甲基化和转录组分析显示，ET 特异性差异表达基因，并且主要是独立特征的，ET 特异性差异甲基化区域。ET 功能的丧失导致多种发育缺陷。在紫外线胁迫后，ET 突变体中嘧啶二聚体的积累表明 ET 在 DNA 修复中具有功能。
