Kino Y, Eto T, Ohtomo N, Hayashi Y, Yamamoto M, Mori R
Microbiol Immunol. 1985;29(2):143-9. doi: 10.1111/j.1348-0421.1985.tb00812.x.
To investigate the protective ability of monoclonal antibodies (MCAs) to viral glycoprotein in herpes simplex virus (HSV) infection, athymic nude mice were inoculated intracutaneously with HSV type-1 (HSV-1) in the midflank. Three hours after inoculation, one group of mice was passively immunized with one of a series of MCAs to glycoprotein gB of HSV-1, and a control group of mice was given phosphate buffered saline alone. The control mice died within 16 days after infection, whereas the mice passively immunized with any of the MCA showed suppressed development of skin lesions. Three of six mice given MCA failed to develop any visible lesions and no HSV could be isolated from the lumbar dorsal root ganglia of these mice 60 days after the challenge. BALB/c mice were also protected from infection with HSV type 2 by passive immunization with MCA to HSV-1 gB.
为研究单克隆抗体（MCAs）对单纯疱疹病毒（HSV）感染中病毒糖蛋白的保护能力，将无胸腺裸鼠在侧腹中部皮内接种1型单纯疱疹病毒（HSV-1）。接种后3小时，一组小鼠用一系列针对HSV-1糖蛋白gB的单克隆抗体之一进行被动免疫，对照组小鼠仅给予磷酸盐缓冲盐水。对照小鼠在感染后16天内死亡，而用任何一种单克隆抗体进行被动免疫的小鼠皮肤损伤的发展受到抑制。给予单克隆抗体的6只小鼠中有3只未出现任何可见损伤，在攻击后60天，从这些小鼠的腰背部脊神经节中未分离到HSV。通过用针对HSV-1 gB的单克隆抗体进行被动免疫，BALB/c小鼠也受到保护，免受2型单纯疱疹病毒感染。
