Mohamed Fakry F, Ktob Gamelat K F, Ismaeil Mohamed E A, Ali Ahmed A H, Goyal Sagar M
Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia 44511, Egypt.
Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA.
Int J Vet Sci Med. 2018 Apr 13;6(1):48-52. doi: 10.1016/j.ijvsm.2018.04.005. eCollection 2018 Jun.
Bovine norovirus (BNoV) has emerged as a viral pathogen that causes a gastrointestinal illness and diarrhea in cattle. Despite its worldwide distribution, very little information is known about BNoV in Africa. In this study, BNoV was detected in 27.6% (8/29) of tested fecal materials, collected from sporadic cases of diarrheic calves, using the reverse transcription-polymerase chain reaction (RT-PCR) and primers that target RNA dependent RNA polymerase gene. Additionally, one primer pair was designed to flank the BNoV-VP2 (small capsid protein) gene for molecular analysis. Study VP2 sequences were phylogenetically-related to BNoV-GIII.2 (Newbury2-like) genotype, which is highly prevalent all over the world. However, they were separated within the cluster and one strain (41FR) grouped with recombinant GIII.P1/GIII.2 strains. Compared to reference VP2 sequences, 14 amino acid substitution mutations were found to be unique to our strains. The study confirms that BNoV is currently circulating among diarrheic calves of Egypt and also characterizes its ORF3 (VP2) genetically. The status of BNoV should be continuously evaluated in Egypt for effective prevention and control.
牛诺如病毒（BNoV）已成为一种导致牛胃肠道疾病和腹泻的病毒病原体。尽管它在全球范围内都有分布，但在非洲关于BNoV的信息却知之甚少。在本研究中，使用逆转录-聚合酶链反应（RT-PCR）和靶向依赖RNA的RNA聚合酶基因的引物，从散发性腹泻犊牛的粪便样本中检测到27.6%（8/29）的样本含有BNoV。此外，设计了一对引物用于扩增BNoV-VP2（小衣壳蛋白）基因侧翼区域以进行分子分析。研究中的VP2序列在系统发育上与BNoV-GIII.2（纽伯里2样）基因型相关，该基因型在全球广泛流行。然而，它们在该聚类中是分开的，并且一个菌株（41FR）与重组GIII.P1/GIII.2菌株聚在一起。与参考VP2序列相比，发现我们的菌株有14个氨基酸替代突变是独特的。该研究证实BNoV目前在埃及腹泻犊牛中传播，并对其ORF3（VP2）进行了基因特征分析。为了有效预防和控制，应持续评估埃及的BNoV状况。
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