Ma Zhigang, Jiang Qian, Quan Chenxi, Liu Lu, Zhang Zhonghua, Xie Jinxing, Zhao Lu, Zhong Qi, Yao Gang, Ma Xuelian
College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi Xinjiang, 830052, China.
Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animal (XJ-KLNDSCHA), Xinjiang Agricultural University, Urumqi Xinjiang, 830052, China.
J Vet Res. 2024 Mar 23;68(1):1-8. doi: 10.2478/jvetres-2024-0005. eCollection 2024 Mar.
Viruses are among the main pathogens causing diarrhoea in calves. The current study found that bovine norovirus (BNoV) is one of the principal viruses causing diarrhoea in calves in Xinjiang, China.
A total of 974 calf faecal samples from six regions in Xinjiang were tested for BNoV using reverse-transcriptase PCR. The genomic characteristics of BNoV and the genetic evolution of the VP1 gene, protein three-dimensional structure characteristics and amino acid variation were analysed using bioinformatics methods.
Epidemiological survey results showed that the infection rate of BNoV was 19.82%, and all samples tested positive in five regions. The results of the genetic evolution analysis showed that BNoV strains from Tacheng of northern Xinjiang and Kashgar of southern Xinjiang both belonged to the GIII.2 genotype of BNoV but were not on the same cluster of evolutionary branches. Additionally, the amino acid variation of the VP1 protein was not observed to significantly affect its spatial structure.
This study is the first to report the genetic characteristics of the BNoV complete genome sequence in Xinjiang and provides a scientific basis for BNoV vaccine development and pathogenesis research.
病毒是引起犊牛腹泻的主要病原体之一。当前研究发现，牛诺如病毒（BNoV）是中国新疆犊牛腹泻的主要病毒之一。
使用逆转录聚合酶链反应对来自新疆六个地区的974份犊牛粪便样本进行BNoV检测。运用生物信息学方法分析BNoV的基因组特征、VP1基因的遗传进化、蛋白质三维结构特征及氨基酸变异。
流行病学调查结果显示，BNoV感染率为19.82%，五个地区的所有检测样本均呈阳性。遗传进化分析结果表明，来自新疆北部塔城和南部喀什的BNoV毒株均属于BNoV的GIII.2基因型，但不在同一进化分支簇上。此外，未观察到VP1蛋白的氨基酸变异对其空间结构有显著影响。
本研究首次报道了新疆BNoV全基因组序列的遗传特征，为BNoV疫苗研发和发病机制研究提供了科学依据。
