Malpartida F, Hopwood D A
Mol Gen Genet. 1986 Oct;205(1):66-73. doi: 10.1007/BF02428033.
We determined the physical and transcriptional organisation of the set of previously cloned biosynthetic genes involved in the production of the polyketide antibiotic actinorhodin by Streptomyces coelicolor A3(2). Complementation and mutational cloning analyses (in part using new phi C31 phage vectors incorporating a transcriptional terminator to block transcription from vector promoters into the cloned DNA) indicate that all the biosynthetic genes, including at least one regulatory (activator) gene, are clustered in a chromosomal region of about 26 kb. The genes are organised in at least four separate transcription units, ranging in size from 1 kb for the class III gene, to a polycistronic transcript of at least 5 kb for the class I, VII and IV genes. Indirect evidence shows that resistance to actinorhodin is also determined by the cloned DNA.
我们确定了天蓝色链霉菌A3(2)中参与聚酮类抗生素放线紫红素生物合成的一组先前克隆的生物合成基因的物理和转录组织。互补和突变克隆分析（部分使用了新的φC31噬菌体载体，该载体包含一个转录终止子以阻止从载体启动子向克隆DNA的转录）表明，所有生物合成基因，包括至少一个调控（激活）基因，都聚集在约26 kb的染色体区域。这些基因至少被组织成四个独立的转录单元，大小从III类基因的1 kb到I类、VII类和IV类基因的至少5 kb的多顺反子转录本不等。间接证据表明，对放线紫红素的抗性也由克隆的DNA决定。
