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1
Kinetics of the proton-deuteron exchange at position H8 of adenine and guanine in DNA.DNA中腺嘌呤和鸟嘌呤H8位置的质子-氘核交换动力学。
Nucleic Acids Res. 1986 Dec 9;14(23):9491-508. doi: 10.1093/nar/14.23.9491.
2
Conformational peculiarities of polynucleotides with a nonrandom base sequence according to the 1H----3H exchange rate in C8H groups of purinic residues.根据嘌呤残基C8H基团中1H→3H的交换率，具有非随机碱基序列的多核苷酸的构象特性。
J Biomol Struct Dyn. 1987 Dec;5(3):601-14. doi: 10.1080/07391102.1987.10506415.
3
Adenine and guanine 8CH exchange in nucleic acids: resolution and measurement by Raman optical multichannel analysis.核酸中腺嘌呤和鸟嘌呤的8CH交换：通过拉曼光学多通道分析进行解析和测量。
Biopolymers. 1990;29(10-11):1465-77. doi: 10.1002/bip.360291013.
4
Internal motions in B- and Z-form poly(dG-dC).poly(dG-dC): 1H NMR relaxation studies.B型和Z型聚（dG-dC）·聚（dG-dC）中的内部运动：1H NMR弛豫研究
Biochemistry. 1985 Oct 22;24(22):6200-11. doi: 10.1021/bi00343a026.
5
Dynamics of DNA duplexes containing internal G.T, G.A, A.C, and T.C pairs: hydrogen exchange at and adjacent to mismatch sites.包含内部G.T、G.A、A.C和T.C碱基对的DNA双链体的动力学：错配位点及其相邻位置的氢交换。
Fed Proc. 1984 Aug;43(11):2663-70.
6
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Biochem Pharmacol. 1988 Mar 15;37(6):1061-6. doi: 10.1016/0006-2952(88)90511-4.
8
Slow exchanging protons in the Z-form of G-C and A-C alternating polymers by using a rapid dialysis method.通过快速透析法在G-C和A-C交替聚合物的Z型中缓慢交换质子。
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23Na NMR relaxation study of the effects of conformation and base composition on the interactions of counterions with double-helical DNA.23Na核磁共振弛豫研究构象和碱基组成对抗衡离子与双螺旋DNA相互作用的影响。
Biochemistry. 1984 Sep 11;23(19):4309-17. doi: 10.1021/bi00314a009.
10
"Asymmetric" opening reaction mechanism of Z-DNA base pairs: a hydrogen exchange study.Z-DNA碱基对的“不对称”开放反应机制：一项氢交换研究。
Biochemistry. 1985 Jul 2;24(14):3603-9. doi: 10.1021/bi00335a031.

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1
Structure and stability of variants of the sarcin-ricin loop of 28S rRNA: NMR studies of the prokaryotic SRL and a functional mutant.28S核糖体RNA的肌动蛋白-蓖麻毒素环变体的结构与稳定性：原核生物SRL及其功能突变体的核磁共振研究
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2
OR3 operator of bacteriophage lambda in a 23 base-pair DNA fragment: sequence-specific 1H NMR assignments for the non-labile protons and comparison with the isolated 17 base-pair operator.噬菌体λ的OR3操纵子位于一个23个碱基对的DNA片段中：非不稳定质子的序列特异性¹H NMR归属以及与分离出的17个碱基对操纵子的比较
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3
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Salt-induced conformational changes of poly(dA-dT).盐诱导的聚（dA-dT）构象变化。
Nucleic Acids Res. 1980 Sep 11;8(17):3965-73. doi: 10.1093/nar/8.17.3965.
2
Molecular conformations and 8-CH exchange rates of purine ribo- and deoxyribonucleotides: investigation by Raman spectroscopy.嘌呤核糖核苷酸和脱氧核糖核苷酸的分子构象及8位碳氢交换率：拉曼光谱研究
Biopolymers. 1984 Jun;23(6):1011-24. doi: 10.1002/bip.360230604.
3
Sequential resonance assignments in 1H NMR spectra of oligonucleotides by two-dimensional NMR spectroscopy.通过二维核磁共振光谱对寡核苷酸的1H NMR光谱进行顺序共振归属。
Biochemistry. 1984 Mar 27;23(7):1371-6. doi: 10.1021/bi00302a006.
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Two-dimensional proton nuclear magnetic resonance investigation of the synthetic deoxyribonucleic acid decamer d(ATATCGATAT)2.合成脱氧核糖核酸十聚体d(ATATCGATAT)₂的二维质子核磁共振研究
Biochemistry. 1983 Dec 6;22(25):5943-51. doi: 10.1021/bi00294a038.
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Sequence-specific recognition of DNA: assignment of nonexchangeable proton resonances in the consensus Pribnow promoter DNA sequence by two-dimensional NMR.DNA的序列特异性识别：通过二维核磁共振确定共有普里布诺启动子DNA序列中不可交换质子的共振归属
Biochemistry. 1984 May 8;23(10):2262-8. doi: 10.1021/bi00305a027.
6
Assignment of the non-exchangeable proton resonances of d(C-G-C-G-A-A-T-T-C-G-C-G) using two-dimensional nuclear magnetic resonance methods.使用二维核磁共振方法对d（C-G-C-G-A-A-T-T-C-G-C-G）的非交换质子共振进行归属
J Mol Biol. 1983 Dec 15;171(3):319-36. doi: 10.1016/0022-2836(83)90096-7.
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Two-dimensional 1H NMR study of the lambda operator site OL1: a sequential assignment strategy and its application.λ 操纵基因位点 OL1 的二维¹H NMR 研究：一种序列归属策略及其应用
Proc Natl Acad Sci U S A. 1984 Jan;81(1):130-4. doi: 10.1073/pnas.81.1.130.
8
A two-dimensional NMR study of the solution structure of a DNA dodecamer comprising the concensus sequence for the specific DNA-binding sites of the glucocorticoid receptor protein.对包含糖皮质激素受体蛋白特异性DNA结合位点共有序列的DNA十二聚体溶液结构的二维核磁共振研究。
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Comparison of the conformation of poly(dI-dC) with poly(dI-dbr5C) and the B and Z forms of poly(dG-dC). One- and two-dimensional NMR studies.聚（dI-dC）与聚（dI-dbr5C）的构象以及聚（dG-dC）的B型和Z型的比较。一维和二维核磁共振研究。
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Preparation of oriented DNA by wet spinning.通过湿纺制备取向DNA。
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DNA中腺嘌呤和鸟嘌呤H8位置的质子-氘核交换动力学。

Kinetics of the proton-deuteron exchange at position H8 of adenine and guanine in DNA.

作者信息

Brandes R, Ehrenberg A

出版信息

Nucleic Acids Res. 1986 Dec 9;14(23):9491-508. doi: 10.1093/nar/14.23.9491.

DOI:10.1093/nar/14.23.9491
PMID:3025816
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311972/
Abstract

Proton-NMR has been used to determine the activation energies and pre-exponential factors for the deuterium exchange of AH8 in poly(dA-dT).poly(dA-dT), and for GH8 in poly(dG-dC).poly(dG-dC). No simple relationship between the kinetic parameters and molecular conformation was found. By addition of 4.5 M NaCl a transition from the B to the Z conformation was induced for poly(dG-dC).poly(dG-dC), and an increased exchange rate was observed. The exchange rate for poly(dA-dT).poly(dA-dT) also increased below 64 degrees C, and a significant decrease in activation energy on addition of 4.5 M NaCl was observed. The exchange rates at T = 55.8 degrees C were also measured for the AH8 and GH8 in random sequence calf thymus DNA. From the difference in exchange rates, a method of preferential labeling of either the AH8 or the GH8 in high molecular weight DNA is evaluated.

摘要

质子核磁共振已被用于测定聚（dA-dT）·聚（dA-dT）中AH8以及聚（dG-dC）·聚（dG-dC）中GH8的氘交换反应的活化能和指前因子。未发现动力学参数与分子构象之间存在简单关系。通过添加4.5M NaCl，聚（dG-dC）·聚（dG-dC）会从B构象转变为Z构象，并观察到交换速率增加。聚（dA-dT）·聚（dA-dT）的交换速率在64℃以下也会增加，并且观察到添加4.5M NaCl后活化能显著降低。还测量了随机序列小牛胸腺DNA中AH8和GH8在T = 55.8℃时的交换速率。根据交换速率的差异，评估了一种对高分子量DNA中的AH8或GH8进行优先标记的方法。