DNA中腺嘌呤和鸟嘌呤H8位置的质子-氘核交换动力学。
Kinetics of the proton-deuteron exchange at position H8 of adenine and guanine in DNA.
作者信息
Brandes R, Ehrenberg A
出版信息
Nucleic Acids Res. 1986 Dec 9;14(23):9491-508. doi: 10.1093/nar/14.23.9491.
Abstract
Proton-NMR has been used to determine the activation energies and pre-exponential factors for the deuterium exchange of AH8 in poly(dA-dT).poly(dA-dT), and for GH8 in poly(dG-dC).poly(dG-dC). No simple relationship between the kinetic parameters and molecular conformation was found. By addition of 4.5 M NaCl a transition from the B to the Z conformation was induced for poly(dG-dC).poly(dG-dC), and an increased exchange rate was observed. The exchange rate for poly(dA-dT).poly(dA-dT) also increased below 64 degrees C, and a significant decrease in activation energy on addition of 4.5 M NaCl was observed. The exchange rates at T = 55.8 degrees C were also measured for the AH8 and GH8 in random sequence calf thymus DNA. From the difference in exchange rates, a method of preferential labeling of either the AH8 or the GH8 in high molecular weight DNA is evaluated.
摘要
质子核磁共振已被用于测定聚（dA-dT）·聚（dA-dT）中AH8以及聚（dG-dC）·聚（dG-dC）中GH8的氘交换反应的活化能和指前因子。未发现动力学参数与分子构象之间存在简单关系。通过添加4.5M NaCl，聚（dG-dC）·聚（dG-dC）会从B构象转变为Z构象，并观察到交换速率增加。聚（dA-dT）·聚（dA-dT）的交换速率在64℃以下也会增加，并且观察到添加4.5M NaCl后活化能显著降低。还测量了随机序列小牛胸腺DNA中AH8和GH8在T = 55.8℃时的交换速率。根据交换速率的差异，评估了一种对高分子量DNA中的AH8或GH8进行优先标记的方法。
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