Mirau P A, Kearns D R
Biochemistry. 1984 Nov 6;23(23):5439-46. doi: 10.1021/bi00318a010.
One- and two-dimensional nuclear Overhauser effects (2D NOE) have been used to compare the conformational properties of 60-80 base pair long duplexes of the synthetic DNA polymer poly(dI-dC) with those of poly(dI-dbr5C) and poly(dG-dC) in the B and Z conformations. Cross peaks in the 2D NOE spectra arising from proton-proton dipolar interactions which are more or less independent of the DNA conformation are used to assign the spectra of these molecules. Other cross peaks are sensitive to the conformational details, and these are used to make deductions about the average conformation in solution. The proton-proton interactions that give rise to the cross peaks in the 2D NOE spectrum of poly(dI-dC) are indicative of a B family conformation and rule out the possibility of some alternative conformations, including A, Z, alternating B, and left-handed B-DNA. The spectra are similar to those obtained from B-form poly(dI-dbr5C) and poly(dG-dC) but different from Z-form poly(dG-dC). Taken together, these results indicate that the solution conformation of poly(dI-dC) is not unusual but more closely resembles that of other B-form DNAs.
一维和二维核Overhauser效应（2D NOE）已被用于比较合成DNA聚合物聚（dI-dC）60 - 80个碱基对长的双链体与聚（dI-dbr5C）和聚（dG-dC）在B和Z构象下的构象性质。二维NOE谱中由质子 - 质子偶极相互作用产生的交叉峰，其或多或少与DNA构象无关，用于确定这些分子的谱图。其他交叉峰对构象细节敏感，这些交叉峰用于推断溶液中的平均构象。在聚（dI-dC）的二维NOE谱中产生交叉峰的质子 - 质子相互作用表明其为B族构象，并排除了一些其他构象的可能性，包括A、Z、交替B和左手B - DNA。这些谱图与从B型聚（dI-dbr5C）和聚（dG-dC）获得的谱图相似，但与Z型聚（dG-dC）不同。综上所述，这些结果表明聚（dI-dC）的溶液构象并非异常，而是更类似于其他B型DNA的构象。
