School of Life Science, Henan University, Dongjing Street, Jinming District, 475004, Kaifeng, Henan province, People's Republic of China.
College of Chemistry and Chemical Engineering, Henan University, Kaifeng, 475004, Henan, China.
Appl Biochem Biotechnol. 2019 Apr;187(4):1475-1487. doi: 10.1007/s12010-018-2874-4. Epub 2018 Sep 26.
The development of bio-fertilizer inoculants is important and desirable. Two phosphate-solubilizing Bacillus subtilis strains were inoculated onto maize bran residue (MBR), which was used as bio-fertilizer carrier and a primary source of nutrients in a medium used for semi-solid fermentation. Water holding capacity, swelling capacity, scanning electron microscopy, and shelf-life assays demonstrated that ground MBR had satisfactory properties for a bio-fertilizer carrier. The maximal soluble phosphorus (P) reached 642.7 ± 0.43 mg l in an orthogonal test under the following optimal conditions: a pH of 7.0, a cultivation temperature of 31 °C, a medium water content of 160%, and a filling capacity of 500 g l. The bio-fertilizer produced by MBR improved the growth of wheat (Triticum aestivum L.) and Chinese cabbage (Brassica rapa pekinensis) with respect to plant height (by up to 18.36%) and the lengths of roots (by up to 34.03%, 27.22%, separately) in a pot experiment. This study integrated the production and storage of a bio-fertilizer to realize the one-step production of a solid bio-fertilizer using MBR.
生物肥料接种剂的开发非常重要且值得期待。将两株解磷枯草芽孢杆菌接种到玉米麸皮残渣（MBR）上，作为生物肥料载体和半固态发酵培养基中的主要营养源。保水能力、溶胀能力、扫描电子显微镜和保质期试验表明，粉碎后的 MBR 作为生物肥料载体具有良好的性能。在 pH 值为 7.0、培养温度为 31°C、培养基含水量为 160%和填充量为 500 g/L 的正交试验条件下，最大可溶性磷（P）达到 642.7±0.43 mg/L。在盆栽试验中，MBR 生产的生物肥料提高了小麦（Triticum aestivum L.）和白菜（Brassica rapa pekinensis）的生长，表现在株高（最高可达 18.36%）和根长（最高可达 34.03%、27.22%）。本研究将生物肥料的生产和储存相结合，实现了使用 MBR 一步法生产固体生物肥料。
