Zhou Ping, Lin Bing, Wang Peng, Pan Tao, Wang Shun, Chen Weisi, Cheng Shaowen, Liu Sha
Department of Radiotherapy, The First Affiliated Hospital, Hainan Medical University, #33 Longhua Road, Longhua District, Haikou, Hainan Province, PR China.
Department of Radiation Physics Center, The First Affiliated Hospital, Hainan Medical University, #33 Longhua Road, Longhua District, Haikou, Hainan Province, PR China.
J Radiat Res. 2019 Jan 1;60(1):17-22. doi: 10.1093/jrr/rry074.
This study aimed to determine the healing effect of hydrogen-rich water (HRW) on radiotherapy-induced skin injury. Rats were irradiated with a 6 MeV electron beam from a Varian linear accelerator. After skin wound formation, rats were individually administrated with distilled water, HRW (1.0 ppm) or HRW (2.0 ppm). We measured the healing time and observed the healing rate of the wounded surface. After irradiation, the malondialdehyde (MDA) content and the superoxide dismutase (SOD) activity in the wounded tissues were evaluated, as determined using an MDA and SOD assay kit. Interleukin-6 (IL-6) and epidermal growth factor (EGF) levels were assessed by enzyme-linked immunosorbent assay (ELISA). Models of skin damage were successfully established using a 44 Gy electronic beam. The healing time was shortened in the two HRW-treated groups (P < 0.05). Furthermore, interventions of HRW resulted in a marked reduction in the MDA (P < 0.05) and IL-6 levels (P < 0.01). Additionally, the SOD activity in the two HRW-treated groups was higher than that in the distilled water group at the end of the 1st, 2nd and 3rd weeks (P < 0.001). The EGF level was also significantly increased at the end of the 1st and 2nd weeks (P < 0.05). Compared with the HRW (1.0 ppm) group, the healing rate was higher and the healing time was reduced in the HRW (2.0 ppm) group. A significant decrease was observed in the IL-6 level at the end of the 1st, 3rd and 4th weeks (P < 0.05) and in the EGF content at the end of the 1 week after the HRW administration (P < 0.01). Collectively, our data indicate that HRW accelerates wound healing of radiation-induced skin lesions through anti-oxidative and anti-inflammatory effects, suggesting that HRW has a healing effect on acute radiation-mediated skin injury, and that this is dependent on the concentration of the hydrogen.
本研究旨在确定富氢水（HRW）对放疗引起的皮肤损伤的愈合效果。用Varian直线加速器产生的6 MeV电子束对大鼠进行照射。在形成皮肤伤口后，分别给大鼠灌胃蒸馏水、1.0 ppm的HRW或2.0 ppm的HRW。我们测量了愈合时间并观察了创面的愈合率。照射后，使用丙二醛（MDA）和超氧化物歧化酶（SOD）检测试剂盒评估创面组织中的丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性。通过酶联免疫吸附测定（ELISA）评估白细胞介素-6（IL-6）和表皮生长因子（EGF）水平。使用44 Gy电子束成功建立了皮肤损伤模型。两个HRW治疗组的愈合时间缩短（P < 0.05）。此外，HRW干预导致MDA（P < 0.05）和IL-6水平显著降低（P < 0.01）。另外，在第1、2和3周结束时，两个HRW治疗组的SOD活性高于蒸馏水组（P < 0.001）。在第1和2周结束时，EGF水平也显著升高（P < 0.05）。与1.0 ppm的HRW组相比，2.0 ppm的HRW组愈合率更高且愈合时间缩短。在第1、3和4周结束时观察到IL-6水平显著降低（P < 0.05），在给予HRW后1周结束时EGF含量显著降低（P < 0.01）。总体而言，我们的数据表明，HRW通过抗氧化和抗炎作用加速放射性皮肤损伤的伤口愈合，表明HRW对急性辐射介导的皮肤损伤具有愈合作用，且这取决于氢的浓度。
