Qi Dong-Dong, Ding Meng-Yuan, Wang Ting, Hayat Muhammad Abid, Liu Tao, Zhang Jian-Tao
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin 150030, China.
Vet Sci. 2021 Nov 4;8(11):264. doi: 10.3390/vetsci8110264.
This study explored the effects of drinking Hydrogen-rich water (HRW) on skin wound healing in dogs. Eight circular wounds were analyzed in each dog. The experimental group was treated with HRW thrice daily, while the control group was provided with distilled water (DW). The wound tissues of dogs were examined histopathologically. The fibroblasts, inflammatory cell infiltration, the average number of new blood vessels, and the level of malondialdehyde (MDA) and superoxide dismutase (SOD) activity in the skin homogenate of the wound was measured using the corresponding kits. The expressions of Nrf-2, HO-1, NQO-1, VEGF, and PDGF were measured using the real-time fluorescence quantitative method. We observed that HRW wounds showed an increased rate of wound healing, and a faster average healing time compared with DW. Histopathology showed that in the HRW group, the average thickness of the epidermis was significantly lower than the DW group. The average number of blood vessels in the HRW group was higher than the DW group. The MDA levels were higher in the DW group than in the HRW group, but the SOD levels were higher in the HRW group than in the DW group. The results of qRT-PCR showed that the expression of each gene was significantly different between the two groups. HRW treatment promoted skin wound healing in dogs, accelerated wound epithelization, reduced inflammatory reaction, stimulated the expression of cytokines related to wound healing, and shortened wound healing time.
本研究探讨了饮用富氢水（HRW）对犬皮肤伤口愈合的影响。对每只犬的8个圆形伤口进行分析。实验组每天用HRW治疗3次，而对照组则给予蒸馏水（DW）。对犬的伤口组织进行组织病理学检查。使用相应试剂盒测量伤口皮肤匀浆中的成纤维细胞、炎症细胞浸润、新生血管平均数量以及丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性。使用实时荧光定量法测量Nrf-2、HO-1、NQO-1、VEGF和PDGF的表达。我们观察到，与DW相比，HRW处理的伤口愈合率提高，平均愈合时间更快。组织病理学显示，HRW组的表皮平均厚度明显低于DW组。HRW组的血管平均数量高于DW组。DW组的MDA水平高于HRW组，但HRW组的SOD水平高于DW组。qRT-PCR结果表明，两组间各基因的表达存在显著差异。HRW处理促进了犬皮肤伤口的愈合，加速了伤口上皮化，减轻了炎症反应，刺激了与伤口愈合相关的细胞因子表达，并缩短了伤口愈合时间。
