Priyanka Uddandarao, Lens Piet Nl
Department of Microbiology and Ryan Institute National University of Ireland Galway Ireland.
J Chem Technol Biotechnol. 2023 Mar;98(3):602-614. doi: 10.1002/jctb.7153. Epub 2022 Aug 11.
The demand for value-added compounds such as amylase, pyruvate and phenolic compounds produced by biological methods has prompted the rapid development of advanced technologies for their enhanced production. Nanobiohybrids (NBs) make use of both the microbial properties of whole-cell microorganisms and the light-harvesting efficiency of semiconductors. Photosynthetic NBs were constructed that link the biosynthetic pathways of with CuS nanoparticles.
In this work, NB formation was confirmed by negative values of the interaction energy, i.e., 2.31 × 10 to -5.52 × 10 kJ mol for CuS-Che NBs, whereas for CuS-Bio NBs the values were -2.31 × 10 to -4.62 × 10 kJ mol for CuS-Bio NBs with spherical nanoparticle interaction. For CuS-Bio NBs with nanorod interaction it ranged from 2.3 × 10 to -3.47 × 10 kJ mol Further, the morphological changes observed by scanning electron microscopy showed the presence of the elements Cu and S in the energy-dispersive X-ray spectra and the presence of CuS bonds in Fourier transform infrared spectroscopy indicate NB formation. In addition, the quenching effect in photoluminescence studies confirmed NB formation. Production yields of amylase, phenolic compounds and pyruvate amounted to 11.2 μmol L, 52.5 μmol L and 28 nmol μL, respectively, in -CuS Bio NBs on the third day of incubation in the bioreactor. Moreover, cells-CuS Bio NBs had amino acids and lipid yields of 6.2 mg mL and 26.5 mg L, respectively. Furthermore, probable mechanisms for the enhanced production of amylase, pyruvate and phenolic compounds are proposed.
-CuS NBs were used for the production of the amylase enzyme and value-added compounds such as pyruvate and phenolic compounds. -CuS Bio NBs showed a greater efficiency compared to -CuS Che NBs as the biologically produced CuS nanoparticles had a higher compatibility with cells © 2022 The Authors. published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).
对通过生物方法生产的淀粉酶、丙酮酸和酚类化合物等增值化合物的需求促使了提高其产量的先进技术的快速发展。纳米生物杂交体（NBs）利用了全细胞微生物的微生物特性和半导体的光捕获效率。构建了将 与硫化铜纳米颗粒的生物合成途径联系起来的光合NBs。
在这项工作中，通过相互作用能的负值证实了NB的形成，即对于硫化铜 - Che NBs，相互作用能为2.31×10至 - 5.52×10 kJ·mol，而对于硫化铜 - Bio NBs，对于具有球形纳米颗粒相互作用的硫化铜 - Bio NBs，其值为 - 2.31×10至 - 4.62×10 kJ·mol。对于具有纳米棒相互作用的硫化铜 - Bio NBs，其范围为2.3×10至 - 3.47×10 kJ·mol。此外，扫描电子显微镜观察到的形态变化表明能量色散X射线光谱中存在铜和硫元素，傅里叶变换红外光谱中存在硫化铜键表明形成了NB。此外，光致发光研究中的猝灭效应证实了NB的形成。在生物反应器中培养第三天，-CuS Bio NBs中淀粉酶、酚类化合物和丙酮酸的产量分别达到11.2 μmol·L、52.5 μmol·L和28 nmol·μL。此外，细胞 - CuS Bio NBs的氨基酸和脂质产量分别为6.2 mg·mL和26.5 mg·L。此外，还提出了淀粉酶、丙酮酸和酚类化合物产量提高的可能机制。
-CuS NBs用于生产淀粉酶以及丙酮酸和酚类化合物等增值化合物。-CuS Bio NBs与 -CuS Che NBs相比显示出更高的效率，因为生物产生的硫化铜纳米颗粒与 细胞具有更高的相容性。© 2022作者。由John Wiley & Sons Ltd代表化学工业协会（SCI）出版。
