小鼠粪便中短链脂肪酸的气相色谱检测方案
Gas Chromatography Detection Protocol of Short-chain Fatty Acids in Mice Feces.
作者信息
Zhang Chaozheng, Liu An, Zhang Tianshuang, Li Yi, Zhao Hua
机构信息
Key Laboratory of Ministry of Education Industrial Fermentation Microbiology, Tianjin Key Laboratory of Industrial Microbiology, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, P. R. China.
出版信息
Bio Protoc. 2020 Jul 5;10(13):e3672. doi: 10.21769/BioProtoc.3672.
Short-chain fatty acids (SCFAs), which are formed mainly by bacteria fermenting undigested carbohydrates in the colon, they are based on the number of carbon atoms in the carbon chain. Organic fatty acids with less than 6 carbon atoms are called short-chain fatty acids. SCFAs are closely related to various aspects of the human body, so more and more researchers concentrate on SCFAs. This protocol describes, a direct injection gas chromatography detection method with a pretreatment method for extracting SCFA from mice feces by combining acidification. The corresponding sample limit of quantization (LOQ) and limit of detection (LOD) are 0.8-1.0 mg/L and 0.5-0.8 mg/L, respectively. The correlation coefficient of calibration curve is greater than 0.999. The recovery rate of the spiked standard is 80%-102%. This method can be used to analyze and determine SCFAs in mice feces. Therefore, this is an economical, effective and reproducible method for SCFAs measurement in mice samples.
短链脂肪酸（SCFAs）主要由结肠中细菌发酵未消化的碳水化合物形成，它们基于碳链中的碳原子数量。碳原子数少于6个的有机脂肪酸被称为短链脂肪酸。SCFAs与人体的各个方面密切相关，因此越来越多的研究人员专注于SCFAs。本方案描述了一种直接进样气相色谱检测方法，该方法采用酸化结合的预处理方法从小鼠粪便中提取SCFA。相应的定量限（LOQ）和检测限（LOD）分别为0.8 - 1.0 mg/L和0.5 - 0.8 mg/L。校准曲线的相关系数大于0.999。加标标准品的回收率为80% - 102%。该方法可用于分析和测定小鼠粪便中的SCFAs。因此，这是一种用于小鼠样品中SCFAs测量的经济、有效且可重复的方法。
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