谱系追踪研究告诉了我们什么？对造血干细胞起源、动态变化及白血病起始细胞的思考。
What do the lineage tracing studies tell us? Consideration for hematopoietic stem cell origin, dynamics, and leukemia-initiating cells.
作者信息
Azevedo Portilho Nathalia, Kobayashi Michihiro, Yoshimoto Momoko
机构信息
Center for Stem Cell and Regenerative Medicine at Institute of Molecular Medicine, McGovern Medical School, University of Texas Health Science Center at Houston, 1825 Pressler Street, Houston, 77030, USA.
出版信息
Int J Hematol. 2019 Jan;109(1):35-40. doi: 10.1007/s12185-018-2537-9. Epub 2018 Sep 27.
The recent advance of technologies enables us to trace the cell fate in vivo by marking the cells that express the gene of interest or by barcoding them at a single cell level. Various tamoxifen-inducible Cre-recombinase mice combined with Rosa-floxed lines are utilized. In this review, with the results revealed by lineage tracing assays, we re-visit the long-standing debate for the origin of hematopoietic stem cells in the mouse embryo, and introduce the view of native hematopoiesis, and possible leukemic-initiating cells emerged during fetal stages.
近期技术的进步使我们能够通过标记表达感兴趣基因的细胞或在单细胞水平对其进行条形码标记来追踪体内细胞命运。各种与Rosa-floxed品系结合的他莫昔芬诱导型Cre重组酶小鼠被加以利用。在本综述中，结合谱系追踪分析所揭示的结果，我们重新审视了关于小鼠胚胎中造血干细胞起源的长期争论，介绍了天然造血的观点，以及胎儿期出现的可能的白血病起始细胞。
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