Department of Plant Cell Biology, Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University, Göttingen, Germany.
Tree Physiol. 2018 Oct 1;38(10):1588-1597. doi: 10.1093/treephys/tpy088.
The TCP-type transcription factors BRANCHED1 and BRANCHED2 shape plant architecture by suppressing bud outgrowth, with BRANCHED2 only playing a minor role in Arabidopsis. Here, we investigated the function of orthologs of these genes in the model tree Populus. We used CRISPR/Cas9 to generate loss-of-function mutants of previously identified Populus BRANCHED1-1 and BRANCHED2-1 candidate genes. BRANCHED1-1 mutants exhibited strongly enhanced bud outgrowth. BRANCHED2-1 mutants had an extreme bud outgrowth phenotype and possessed two ectopic leaves at each node. While BRANCHED1 function is conserved in poplar, BRANCHED2, in contrast to its Arabidopsis counterpart, plays an even more critical role in bud outgrowth regulation. In addition, we identified a new, not yet reported association of this gene to leaf development.
TCP 类转录因子 BRANCHED1 和 BRANCHED2 通过抑制芽生长来塑造植物的形态结构，其中 BRANCHED2 在拟南芥中只起次要作用。在这里，我们研究了这些基因在模式树种杨树中的同源物的功能。我们使用 CRISPR/Cas9 生成了先前鉴定的杨树 BRANCHED1-1 和 BRANCHED2-1 候选基因的功能丧失突变体。BRANCHED1-1 突变体表现出强烈增强的芽生长。BRANCHED2-1 突变体具有极端的芽生长表型，每个节点都有两个异位叶。虽然 BRANCHED1 在杨树中的功能是保守的，但与拟南芥中的 BRANCHED2 不同，它在芽生长调控中起着更为关键的作用。此外，我们还发现了该基因与叶片发育的一个新的、尚未报道的关联。
