Ugrankar Rupali, Theodoropoulos Pano, Akdemir Fatih, Henne W Mike, Graff Jonathan M
Department of Developmental Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390, USA.
Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390, USA.
Commun Biol. 2018 Aug 13;1:110. doi: 10.1038/s42003-018-0109-4. eCollection 2018.
In mammals, blood glucose levels likely play a role in appetite regulation yet the mechanisms underlying this phenomenon remain opaque. Mechanisms can often be explored from genetic approaches. To determine if circulating sugars might be involved in feeding behaviors, we scored hemolymph glucose and trehalose, and food ingestion in larvae subjected to various diets, genetic mutations, or RNAi. We found that larvae with glucose elevations, hyperglycemia, have an aversion to feeding; however, trehalose levels do not track with feeding behavior. We further discovered that insulins and SLC5A11 may participate in glucose-regulated feeding. To see if food aversion might be an appropriate screening method for hyperglycemia candidates, we developed a food aversion screen to score larvae with abnormal feeding for glucose. We found that many feeding defective larvae have glucose elevations. These findings highlight intriguing roles for glucose in fly biology as a potential cue and regulator of appetite.
在哺乳动物中，血糖水平可能在食欲调节中发挥作用，但其背后的机制仍不明朗。通常可以从基因方法来探究机制。为了确定循环糖类是否参与进食行为，我们对不同饮食、基因突变或RNA干扰处理的幼虫的血淋巴葡萄糖和海藻糖水平以及食物摄入量进行了评分。我们发现血糖升高（即高血糖）的幼虫对进食有厌恶感；然而，海藻糖水平与进食行为无关。我们进一步发现胰岛素和溶质载体家族5成员11（SLC5A11）可能参与葡萄糖调节的进食过程。为了探究食物厌恶是否可能是筛选高血糖候选者的合适方法，我们开发了一种食物厌恶筛选方法来对进食异常的幼虫进行葡萄糖评分。我们发现许多进食有缺陷的幼虫血糖升高。这些发现凸显了葡萄糖在果蝇生物学中作为食欲潜在线索和调节因子的有趣作用。
