Watson J B, Gralla J D
J Virol. 1987 Mar;61(3):748-54. doi: 10.1128/JVI.61.3.748-754.1987.
The association of infecting simian virus 40 with insoluble nuclear structures was assayed by disrupting infected nuclei and assaying insoluble fractions for virus. Three methods were used which lyse nuclei but maintain the insolubility of residual nuclear structures: sonication, high-salt-Triton-EDTA extraction, and low-salt-lithium diiodosalicylate extraction. After each type of nuclear extraction, infecting simian virus 40 remained associated with the residual nuclear structures. This association depended strictly on natural viral infections and on the use of buffers containing moderate amounts of salt and Mg2+ for the isolation of infected nuclei. These viral interactions exhibited behavior similar to host cell DNA interactions studied by analogous assays. Both viral DNA and coat proteins were found associated with the host cell nuclear superstructure. We concluding that at early times after infection the viral templates mimic the state of the host cell chromatin by attaching to the cellular nuclear matrix.
通过破坏受感染的细胞核并检测不溶性部分中的病毒，来测定感染性猿猴病毒40与不溶性核结构的关联。使用了三种方法来裂解细胞核，但保持残留核结构的不溶性：超声处理、高盐 - 曲拉通 - 乙二胺四乙酸提取和低盐 - 二碘水杨酸锂提取。在每种类型的核提取后，感染性猿猴病毒40仍与残留的核结构相关联。这种关联严格依赖于自然病毒感染以及使用含有适量盐和Mg2+的缓冲液来分离受感染的细胞核。这些病毒相互作用表现出与通过类似检测方法研究的宿主细胞DNA相互作用相似的行为。发现病毒DNA和衣壳蛋白都与宿主细胞核超结构相关联。我们得出结论，在感染后的早期，病毒模板通过附着于细胞核基质来模拟宿主细胞染色质的状态。
