芽孢杆菌噬菌体φ29基因14和15的核苷酸序列：基因15与其他噬菌体溶菌酶的同源性
Nucleotide sequence of Bacillus phage phi 29 genes 14 and 15: homology of gene 15 with other phage lysozymes.
作者信息
Garvey K J, Saedi M S, Ito J
出版信息
Nucleic Acids Res. 1986 Dec 22;14(24):10001-8. doi: 10.1093/nar/14.24.10001.
Abstract
The nucleotide sequence of Bacillus phage phi 29 genes 14 (g14) and 15 (g15) have been determined and shown to encode proteins with molecular weights of 15,014 and 28,022, respectively. The g14 open reading frame (ORF) was confirmed by sequencing a sus14(1241) mutant. Gene product 15 (gp15) has considerable homology with Salmonella phage P22 lysozyme and lesser homology with Escherichia coli phage T4 lysozyme. Putative translation signals are identified. In addition, the role of a previously described promoter, B2, is discussed.
摘要
已确定芽孢杆菌噬菌体φ29基因14（g14）和15（g15）的核苷酸序列，结果表明它们分别编码分子量为15,014和28,022的蛋白质。通过对sus14(1241)突变体进行测序，证实了g14开放阅读框（ORF）。基因产物15（gp15）与沙门氏菌噬菌体P22溶菌酶具有相当高的同源性，与大肠杆菌噬菌体T4溶菌酶的同源性较低。已鉴定出推定的翻译信号。此外，还讨论了先前描述的启动子B2的作用。
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