Chen Si, Cheng Huicai, Wyckoff Kristen N, He Qiang
Department of Civil and Environmental Engineering, The University of Tennessee, Knoxville, TN, 37996, USA.
Biology Institute, Hebei Academy of Sciences, Shijiazhuang, Hebei, China.
J Ind Microbiol Biotechnol. 2016 Jun;43(6):771-81. doi: 10.1007/s10295-016-1760-8. Epub 2016 Mar 28.
To identify potential linkages between specific bacterial populations and process performance in anaerobic digestion, the dynamics of bacterial community structure was monitored with high-throughput sequencing in triplicate anaerobic digesters treating animal waste. Firmicutes and Bacteroidetes were found as the two most abundant populations, however, with contrasting population dynamics in response to organic overloading. Firmicutes dominated the bacterial community during stable process performance at low organic loading rate, representing over 50 % of the bacterial abundance. In contrast, the onset of organic overloading raised the relative abundance of Bacteroidetes from 20 ± 2.6 to 44 ± 3.1 %. In addition to the significant negative correlation between the relative abundance of Firmicutes and Bacteroidetes, populations of Firmicutes and Bacteroidetes were found to be linked to process parameters including organic loading rate, volatile fatty acids concentration, and methane production. Therefore, the population abundance ratio of Firmicutes to Bacteroidetes (F/B ratio) was suggested as a potential indicator for process performance. The interactions between Firmicutes and Bacteroidetes populations could be exploited to develop strategies for the prevention of performance perturbation in anaerobic digestion processes.
为了确定厌氧消化过程中特定细菌群体与工艺性能之间的潜在联系，采用高通量测序技术对处理动物粪便的三个重复厌氧消化器中的细菌群落结构动态进行了监测。厚壁菌门和拟杆菌门是两个数量最多的菌门，然而，它们对有机负荷过载的种群动态变化相反。在低有机负荷率下工艺性能稳定期间，厚壁菌门在细菌群落中占主导地位，占细菌丰度的50%以上。相比之下，有机负荷过载的开始使拟杆菌门的相对丰度从20±2.6%提高到44±3.1%。除了厚壁菌门和拟杆菌门的相对丰度之间存在显著的负相关外，还发现厚壁菌门和拟杆菌门的菌门数量与包括有机负荷率、挥发性脂肪酸浓度和甲烷产量在内的工艺参数有关。因此，建议将厚壁菌门与拟杆菌门的种群丰度比（F/B比）作为工艺性能的潜在指标。厚壁菌门和拟杆菌门菌门之间的相互作用可用于制定预防厌氧消化过程中性能扰动的策略。
