Universidad de Guadalajara, Guadalajara, Mexico.
Universidad de la República, Montevideo, Uruguay.
Intervirology. 2018;61(3):105-110. doi: 10.1159/000492425. Epub 2018 Oct 2.
We aimed to detect and characterize hepatitis E virus (HEV) RNA in sera samples from a pediatric population infected with the hepatitis A virus (HAV) exhibiting acute hepatitis and to correlate the infection status with the clinical outcome.
Seventy-five ELISA-positive samples from children containing anti-HAV and anti-HEV IgM were used to amplify and characterize partial regions within HEV ORF2. A statistical comparison of clinical data between HEV IgM-positive/HEV RNA-positive patients and HEV IgM-positive/HEV RNA-negative patients was performed.
Thirteen out of 75 IgM-positive samples provided amplification of discrete regions of the HEV genome. Nested RT-PCR-based detection and subsequent sequencing of 5 samples confirmed the identity of HEV genotype 1 (G1), which had not been previously reported in Mexico. Though not significant, a trend towards exacerbated clinical manifestations was found in HEV RNA-positive patients relative to HEV RNA-negative patients.
An elevated rate of G1 RNA was detected. Hepatitis E seems to be a neglected disease in Mexico and epidemic strains of HEV are likely to play a role as causative agents of acute hepatitis in highly exposed children. Although HAV is endemic in Mexico, an HEV-RNA detection rate of 17% in co-infected samples shows the need for screening for HEV as a part of future vaccination strategies.
我们旨在检测和鉴定甲型肝炎病毒（HAV）感染的急性肝炎患儿血清样本中的戊型肝炎病毒（HEV）RNA，并将感染状态与临床结果相关联。
使用 75 份 ELISA 阳性的抗-HAV 和抗-HEV IgM 阳性的儿童样本，对 HEV ORF2 内的部分区域进行扩增和特征分析。对 HEV IgM 阳性/HEV RNA 阳性患者和 HEV IgM 阳性/HEV RNA 阴性患者的临床数据进行统计学比较。
13 份 IgM 阳性样本中有 13 份提供了离散的 HEV 基因组区域的扩增。5 份样本的巢式 RT-PCR 检测和后续测序证实了之前在墨西哥尚未报道的 HEV 基因型 1（G1）的存在。虽然没有显著意义，但与 HEV RNA 阴性患者相比，HEV RNA 阳性患者的临床表现似乎更为严重。
检测到较高比例的 G1 RNA。戊型肝炎在墨西哥似乎是一种被忽视的疾病，流行株的 HEV 可能在高度暴露的儿童中作为急性肝炎的致病因子发挥作用。尽管 HAV 在墨西哥流行，但在合并感染样本中 HEV-RNA 的检出率为 17%，表明需要对 HEV 进行筛查，作为未来疫苗接种策略的一部分。
