Zaĭtsevskaia E V, Babichev V A, Maksimova G F
Farmakol Toksikol. 1977 May-Jun;40(3):322-6.
Through an analysis of the immune response of mice to sheep's erythrocytes within a system of syngenic transfer the action of Methotrexate on the T- and B-lymphocytes was studied. Splenic cells of donors receiving methotrexate were introduced to lethally irradiated recipients together with the bone morrow or thymus cells. In the recipients' spleen the content of antibody-forming cells and hemolytic foci were determined. It was shown that in recipients receiving splenic cells alone the number of antibody-forming cells and of the foci sharply declined under the effect of methotrexate. Introduction of the bone marrow cells contributed to reconstitution of the response in recipients at the level of the antibody-forming cells, but not at that of the hemolytic foci. At the same time, thymocytes increased the content of the antibody-forming cells up to a level exceeding the control one, as well as the number of foci. The results proof methotrexate capable of inhibiting the proliferation of the T- and B-cells in the spleen of mice, induced by the antigen.
通过在同基因转移系统中分析小鼠对绵羊红细胞的免疫反应，研究了甲氨蝶呤对T淋巴细胞和B淋巴细胞的作用。将接受甲氨蝶呤的供体脾细胞与骨髓细胞或胸腺细胞一起引入经致死剂量照射的受体。测定受体脾脏中抗体形成细胞的含量和溶血灶。结果表明，在仅接受脾细胞的受体中，甲氨蝶呤的作用使抗体形成细胞数量和溶血灶数量急剧下降。引入骨髓细胞有助于受体在抗体形成细胞水平上恢复反应，但在溶血灶水平上则不然。同时，胸腺细胞使抗体形成细胞的含量增加到超过对照水平，溶血灶数量也增加。结果证明甲氨蝶呤能够抑制抗原诱导的小鼠脾脏中T细胞和B细胞的增殖。
