Department of Electronic Engineering and Computer Science , University of Michigan , Ann Arbor , Michigan 48109 , United States.
Department of Computational Medicine and Bioinformatics , University of Michigan , Ann Arbor , Michigan 48109 , United States.
J Proteome Res. 2018 Dec 7;17(12):4061-4071. doi: 10.1021/acs.jproteome.8b00442. Epub 2018 Oct 23.
The Chromosome-centric Human Proteome Project (C-HPP), announced in September 2016, is an initiative to accelerate progress on the detection and characterization of neXtProt PE2,3,4 "missing proteins" (MPs) with a mandate to each chromosome team to find about 50 MPs over 2 years. Here we report major progress toward the neXt-MP50 challenge with 43 newly validated Chr 17 PE1 proteins, of which 25 were based on mass spectrometry, 12 on protein-protein interactions, 3 on a combination of MS and PPI, and 3 with other types of data. Notable among these new PE1 proteins were five keratin-associated proteins, a single olfactory receptor, and five additional membrane-embedded proteins. We evaluate the prospects of finding the remaining 105 MPs coded for on Chr 17, focusing on mass spectrometry and protein-protein interaction approaches. We present a list of 35 prioritized MPs with specific approaches that may be used in further MS and PPI experimental studies. Additionally, we demonstrate how in silico studies can be used to capture individual peptides from major data repositories, documenting one MP that appears to be a strong candidate for PE1. We are close to our goal of finding 50 MPs for Chr 17.
染色体中心的人类蛋白质组计划（C-HPP）于 2016 年 9 月宣布，旨在加速检测和描述下一个蛋白（neXtProt PE2,3,4）的进展，该计划要求每个染色体团队在两年内找到约 50 个 MPs。在这里，我们报告了在 neXt-MP50 挑战方面的重大进展，其中包括 43 个新验证的 Chr17PE1 蛋白，其中 25 个基于质谱，12 个基于蛋白质-蛋白质相互作用，3 个基于 MS 和 PPI 的组合，还有 3 个基于其他类型的数据。在这些新的 PE1 蛋白中，有 5 个角蛋白相关蛋白、1 个嗅觉受体和 5 个额外的膜嵌入蛋白。我们评估了在 Chr17 上找到其余 105 个编码 MPs 的前景，重点关注质谱和蛋白质-蛋白质相互作用方法。我们提出了一个有 35 个优先考虑的 MPs 的列表，并提供了可能用于进一步 MS 和 PPI 实验研究的具体方法。此外，我们展示了如何使用计算研究从主要数据存储库中捕获单个肽，记录一个似乎是 PE1 的强有力候选者的 MP。我们已经接近在 Chr17 上找到 50 个 MPs 的目标。
