Spenney J G, Bhown M
Gastroenterology. 1977 Nov;73(5):995-9.
The effects of acetylsalicylic acid on gastric mucosal ATP and phosphocreatine and mucosal O2 consumption were studied using in vitro bullfrog gastric mucosa. Electrophysiological parameters (potential difference and resistance) changed rapidly after exposure to 10 or 20 mM acetylsalicylic acid at pH 3.0, 4.0, or 6.0 in the mucosal solution. ATP and phosphocreatine content decreased rapidly after exposure. Control mucosae contained 11.8 +/- 0.98 nmoles of ATP and 11.5 +/- 0.68 nmoles of phosphocreatine per mg of protein. ATP content decreased 21% and phosphocreatine decreased 45% after 15 to 30 min of exposure. Longer durations of exposure reduced both ATP and phosphocreatine to 10 to 15% of control levels. O2 consumption was increased during the first 15 to 30 min after exposure to acetylsalicylate. Longer durations of exposure reduced O2 consumption to near control levels. Study of isolated mitochondria confirmed that acetylsalicylate itself did not significantly affect mitochondrial respiration, respiratory control ratio, or ADP:O ratio. Salicylate had a biphasic effect which was concentration dependent. Salicylate initially stimulated and later inhibited mitochondrial respiration. Using various substrates the site of inhibition appeared to be located in the segment of the respiratory chain between coenzyme Q and cytochrome c1.
使用离体牛蛙胃黏膜研究了乙酰水杨酸对胃黏膜三磷酸腺苷（ATP）、磷酸肌酸及黏膜耗氧量的影响。在黏膜溶液pH值为3.0、4.0或6.0的条件下，暴露于10或20 mM乙酰水杨酸后，电生理参数（电位差和电阻）迅速改变。暴露后ATP和磷酸肌酸含量迅速下降。对照黏膜每毫克蛋白质含11.8±0.98纳摩尔ATP和11.5±0.68纳摩尔磷酸肌酸。暴露15至30分钟后，ATP含量下降21%，磷酸肌酸下降45%。暴露时间延长会使ATP和磷酸肌酸均降至对照水平的10%至15%。暴露于乙酰水杨酸后的最初15至30分钟内耗氧量增加。暴露时间延长会使耗氧量降至接近对照水平。对分离线粒体的研究证实，乙酰水杨酸本身对线粒体呼吸、呼吸控制率或ADP:O比率无显著影响。水杨酸盐具有浓度依赖性的双相效应。水杨酸盐最初刺激线粒体呼吸，随后抑制线粒体呼吸。使用各种底物时，抑制部位似乎位于辅酶Q和细胞色素c1之间的呼吸链段。
