Acikan Izzet, Mehmet Gul, Artas Gokhan, Yaman Ferhan, Deniz Gulnihal, Bulmus Ozgur, Kom Mustafa, Kirtay Mustafa, Dundar Serkan
Sutcu Imam University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Kahramanmaras, Turkey.
Harran University, Faculty of Dentistry, Department of Periodontology, Sanliurfa, Turkey.
Braz Oral Res. 2018 Sep 21;32:e85. doi: 10.1590/1807-3107bor-2018.vol32.0085.
This study aimed to investigate the effects of different doses of systemic melatonin application on new bone formation during mandibular distraction osteogenesis (DO) in rats. Mandibular DO was performed on 30 adult female Sprague-Dawley rats, which were randomly divided into three groups: control group (CNT), melatonin dose 1 (MLT-D1), and melatonin dose 2 (MLT-D2). A five-day latent waiting period and a ten-day distraction phase followed the surgery. After the surgery, rats from the MLT-D1 and MLT-D2 groups received 25 and 50 mg/kg melatonin, respectively, at 7, 14, 21, 28, and 35 days. The animals were euthanised 28 days after distraction, i.e. at 43 days after surgery. Histological and histomorphometric analyses revealed that the distracted bone area was completely filled with new bone formation in all three groups. The MLT-D2 group exhibited the most new bone formation, followed by MLT-D1 and CNT. The melatonin groups had more osteoclasts than the CNT (p < 0.05). The number of osteoblasts was higher in the melatonin groups than in the CNT group, and the MLT-D2 had more osteoclasts than the MLT-D1 group (p < 0.05). Finally, the osteopontin (OPN) and vascular endothelial growth factor (VEGF) levels were higher in the melatonin groups than in the CNT group, and the MLT-D2 had higher OPN and VEGF levels than the MLT-D1 (p < 0.05). This study suggests that systemic melatonin application could increase new bone formation in DO.
本研究旨在探讨全身应用不同剂量褪黑素对大鼠下颌骨牵张成骨（DO）过程中新骨形成的影响。对30只成年雌性Sprague-Dawley大鼠进行下颌骨DO手术，将其随机分为三组：对照组（CNT）、褪黑素剂量1组（MLT-D1）和褪黑素剂量2组（MLT-D2）。术后有5天的潜伏等待期和10天的牵张期。术后，MLT-D1组和MLT-D2组的大鼠分别在第7、14、21、28和35天接受25和50 mg/kg的褪黑素。牵张28天后，即术后43天，对动物实施安乐死。组织学和组织形态计量学分析显示，所有三组中牵张骨区域均完全被新骨形成填充。MLT-D2组新骨形成最多，其次是MLT-D1组和CNT组。褪黑素组的破骨细胞比CNT组更多（p < 0.05）。褪黑素组的成骨细胞数量高于CNT组，且MLT-D2组的破骨细胞比MLT-D1组更多（p < 0.05）。最后，褪黑素组的骨桥蛋白（OPN）和血管内皮生长因子（VEGF）水平高于CNT组，且MLT-D2组的OPN和VEGF水平高于MLT-D1组（p < 0.05）。本研究表明，全身应用褪黑素可增加DO过程中的新骨形成。
