Abdel-Halim M N
Int J Radiat Biol Relat Stud Phys Chem Med. 1977 Oct;32(4):309-15. doi: 10.1080/09553007714551071.
The effect of gamma-radiation (1800 rad) on polyribosome distribution in the regenerating livers of intact and adrenalectomized rats was studied both before and after partial hepatectomy. The animals were divided into four sub-groups: (1) control; (2) irradiated only; (3) partially hepatectomized; and (4) irradiated partially hepatectomized. The relative distribution of lighter oligosomes to heavier polyribosomes was analysed by sucrose density gradient centrifugation (10-40 per cent). Partial hepatectomy by itself increased the proportion of heavier polyribosomes in both intact and adrenalectomized rats. However, when gamma-rays were delivered 2 hours before or 2 hours after partial-hepatectomy, the formation of heavy polyribosomes was decreased for at least 24 hours after hepatectomy in the adrenal intact rats. This depression was not maintained until later times (48 and 72 hours after hepatectomy) when an increase in the proportion of heavy polyribosomes relative to light oligosomes was observed. In addition, irradiation did not measurably affect the distribution of polyribosomes in regenerating hepatocytes of adrenalectomized rats at any time after partial hepatectomy.
研究了γ射线辐射（1800拉德）对完整大鼠和肾上腺切除大鼠再生肝脏中多核糖体分布的影响，观察时间为部分肝切除术前和术后。动物被分为四个亚组：（1）对照组；（2）仅接受辐射组；（3）部分肝切除组；（4）接受辐射并部分肝切除组。通过蔗糖密度梯度离心法（10%-40%）分析较轻的寡核糖体与较重的多核糖体的相对分布。部分肝切除本身增加了完整大鼠和肾上腺切除大鼠中较重多核糖体的比例。然而，当在部分肝切除术前2小时或术后2小时进行γ射线照射时，肾上腺完整大鼠在肝切除后至少24小时内重多核糖体的形成减少。这种抑制在后期（肝切除后48小时和72小时）未持续存在，此时观察到重多核糖体相对于轻寡核糖体的比例增加。此外，在部分肝切除后的任何时间，辐射对肾上腺切除大鼠再生肝细胞中多核糖体的分布均无明显影响。
