Abdel-Halim M N
Int J Radiat Biol Relat Stud Phys Chem Med. 1979 Mar;35(3):245-51. doi: 10.1080/09553007914550281.
Newly synthesized messenger RNA, as measured by a 40 min uptake of the radioactive precursor (6-14C) orotic acid, was studied in the regenerating livers of non-irradiated and gamma-irradiated (1800 rad) adrenal-intact and adrenalectomized rats 24 aand 48 hours after partial hepatectomy. Two groups of rats, one with and one without adrenal glands, were each divided into four subgroups: (1) control rats, (2) irradiated rats, (3) partially hepatectomized rats and (4) irradiated, partially hepatectomized rats. The radioactive profile of polyribosome formation and distribution was determined by sucrose density gradient centrifugation (10--40 per cent). The result of this study indicates that ionizing radiation decreases the synthesis of newly formed messenger RNA in re generating livers of adrenal-intact rats. However, adrenalectomy largely abolished that inhibition. These data suggest that the decrease in messenger RNA synthesis may be explained by the disturbance of adrenal hormones induced by partial hepatectomy and ionizing radiation.
通过对放射性前体（6-¹⁴C）乳清酸40分钟的摄取来测定新合成的信使核糖核酸（mRNA），在部分肝切除术后24小时和48小时，对未受照射和经γ射线照射（1800拉德）的、肾上腺完整和肾上腺切除的大鼠再生肝脏进行了研究。两组大鼠，一组有肾上腺，一组无肾上腺，每组又各分为四个亚组：（1）对照大鼠，（2）受照射大鼠，（3）部分肝切除大鼠，（4）受照射且部分肝切除大鼠。通过蔗糖密度梯度离心（10%-40%）测定多核糖体形成和分布的放射性图谱。本研究结果表明，电离辐射会降低肾上腺完整大鼠再生肝脏中新形成的信使核糖核酸的合成。然而，肾上腺切除术在很大程度上消除了这种抑制作用。这些数据表明，信使核糖核酸合成的减少可能是由部分肝切除和电离辐射引起的肾上腺激素紊乱所导致的。
