Institute of Evolution, University of Haifa, 199 Abba-Hushi Avenue, Mt. Carmel, 3498838, Haifa, Israel.
Department of Evolutionary and Environmental Biology, University of Haifa, 199 Abba-Hushi Avenue, Mt. Carmel, 3498838, Haifa, Israel.
Nat Commun. 2018 Oct 3;9(1):3735. doi: 10.1038/s41467-018-06138-9.
Yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating fungal disease threatening much of global wheat production. Race-specific resistance (R)-genes are used to control rust diseases, but the rapid emergence of virulent Pst races has prompted the search for a more durable resistance. Here, we report the cloning of Yr15, a broad-spectrum R-gene derived from wild emmer wheat, which encodes a putative kinase-pseudokinase protein, designated as wheat tandem kinase 1, comprising a unique R-gene structure in wheat. The existence of a similar gene architecture in 92 putative proteins across the plant kingdom, including the barley RPG1 and a candidate for Ug8, suggests that they are members of a distinct family of plant proteins, termed here tandem kinase-pseudokinases (TKPs). The presence of kinase-pseudokinase structure in both plant TKPs and the animal Janus kinases sheds light on the molecular evolution of immune responses across these two kingdoms.
条锈病，由条形柄锈菌小麦专化型（Pst）引起，是一种严重威胁全球小麦生产的真菌病害。利用具有特定抗病性的基因（R）来控制锈病，但 Pst 毒性菌株的快速出现促使人们寻求更持久的抗性。在这里，我们报告了 Yr15 的克隆，它是一个源自野生二粒小麦的广谱 R 基因，编码一个假定的激酶-假激酶蛋白，称为小麦串联激酶 1，在小麦中具有独特的 R 基因结构。在植物界的 92 个假定蛋白中存在类似的基因结构，包括大麦 RPG1 和 Ug8 的候选蛋白，这表明它们是植物蛋白的一个独特家族的成员，我们称之为串联激酶-假激酶（TKPs）。植物 TKPs 和动物 Janus 激酶中都存在激酶-假激酶结构，这揭示了这两个领域的免疫反应的分子进化。
