Mann W
Arch Otorhinolaryngol. 1986;243(5):296-303. doi: 10.1007/BF00460205.
A rat animal model was used to study the ultrastructure of submucosal calcifications induced in the middle ear following inoculation with Streptococcus pyogenes and high doses of parenteral vitamin D3. The morphological changes present in affected animals resembled the classical picture of tympanosclerosis. While calcification occurred about bacterial remnants and myelin structures, the most important calcification centers were lysosomal and non-lysosomal matrix vesicles in the extracellular spaces. These formed band-like calcifications close to the basal membrane without affecting the epithelial layer. This animal model offers the possibility of studying the effect of various therapeutic regimens in the treatment of the dynamic tympanosclerotic process.
采用大鼠动物模型研究接种化脓性链球菌和高剂量肠外维生素D3后中耳黏膜下钙化的超微结构。受影响动物出现的形态学变化类似于典型的鼓室硬化表现。虽然钙化发生在细菌残余物和髓鞘结构周围，但最重要的钙化中心是细胞外空间中的溶酶体和非溶酶体基质小泡。这些在靠近基底膜处形成带状钙化，而不影响上皮层。该动物模型为研究各种治疗方案对动态鼓室硬化过程的治疗效果提供了可能性。
