Department of Animal Science, South Dakota State University, Brookings, SD 57007, USA.
Department of Animal Science, University of Minnesota, St. Paul, MN 55108-6118, USA.
Poult Sci. 2019 Mar 1;98(3):1302-1309. doi: 10.3382/ps/pey452.
The objective was to determine effects of adding phytase, amylase, and a cocktail of non-starch polysaccharide-degrading enzymes (NSPase) individually or in combinations to corn-soybean meal-based diet for broilers on apparent ileal digestibility (AID) of nutrients and dietary AMEn value. Four hundred and eighty male broiler chicks were divided into 80 groups and fed 8 diets in a completely randomized design (10 groups/diet) from day 15 to 21 of age. The diets were basal diet unsupplemented or supplemented with phytase (1,500 FTU/kg; Ronozyme HiPhos), amylase (80 KNU/kg, Ronozyme HiStarch), and NSPase (75 g/metric ton, Ronozyme Multigrain) individually or in all possible combinations. The basal diet contained the phytase at 1,000 FTU/kg, and was formulated to meet the NRC (1994) recommended nutrient requirements for broiler chickens except for ME, Ca, and non-phytate P, which were reduced by 150 kcal/kg, 0.18%, and 0.15%, respectively. Addition of phytase to the basal diet increased (P < 0.05) AID of P from 40.4 to 59.3%. Addition of amylase, NSPase or a combination of amylase and NSPase to the phytase-supplemented basal diet further increased (P < 0.05) AID of P to 63.4, 69.9, and 67.3%, respectively. Addition of phytase, amylase or a combination of amylase and NSPase to the basal diet did not affect dietary AMEn value. However, addition of NSPase alone or a combination of phytase and amylase or of phytase and NSPase to the basal diet improved (P < 0.05) dietary AMEn value from 3,203 to 3,339, 3,309 or 3,289 kcal/kg, respectively. In conclusion, it is more beneficial (with regard to AID of P and dietary AMEn) to add amylase and NSPase to phytase-supplemented diets for broilers. Because the basal diet contained phytase at 1,000 FTU/kg, the increase in AID of P due to supplemental phytase (1,500 FTU/kg) indicates that supplemental phytase at 2,500 FTU/kg is more beneficial with regard to improving AID of P than supplemental phytase at 1,000 FTU/kg.
本研究旨在确定在玉米-豆粕基础饲粮中单独或组合添加植酸酶、淀粉酶和非淀粉多糖降解酶（NSPase）混合物对 15-21 日龄肉鸡的养分表观回肠消化率（AID）和饲粮代谢能值（AME）的影响。将 480 只雄性肉鸡雏鸡分为 80 组，采用完全随机设计（每组 10 个重复），15 日龄至 21 日龄期间饲喂 8 种饲粮。饲粮基础饲粮未添加或分别添加植酸酶（1500 FTU/kg，Ronozyme HiPhos）、淀粉酶（80 KNU/kg，Ronozyme HiStarch）和 NSPase（75 g/吨，Ronozyme Multigrain），或组合添加上述酶制剂。基础饲粮中植酸酶添加量为 1000 FTU/kg，按照满足肉鸡的 NRC（1994）推荐营养需要进行配制，除代谢能、钙和非植酸磷外，其他营养素水平均降低 150 kcal/kg、0.18%和 0.15%。在基础饲粮中添加植酸酶提高了磷的 AID（P<0.05），从 40.4%提高到 59.3%。在添加植酸酶的基础饲粮中添加淀粉酶、NSPase 或淀粉酶和 NSPase 的组合进一步提高了磷的 AID（P<0.05），分别提高到 63.4%、69.9%和 67.3%。在基础饲粮中添加植酸酶、淀粉酶或淀粉酶和 NSPase 的组合对饲粮 AMEn 值没有影响。然而，单独添加 NSPase 或组合添加植酸酶和淀粉酶或植酸酶和 NSPase 提高了饲粮 AMEn 值（P<0.05），分别从 3203、3309 或 3289 kcal/kg 提高到 3339、3309 或 3289 kcal/kg。综上所述，在添加植酸酶的饲粮中添加淀粉酶和 NSPase 更有利于（提高磷的 AID 和饲粮 AMEn 值）。由于基础饲粮中含有 1000 FTU/kg 的植酸酶，因此添加 1500 FTU/kg 的植酸酶提高了磷的 AID（P<0.05），这表明添加 2500 FTU/kg 的植酸酶比添加 1000 FTU/kg 的植酸酶更有利于提高磷的 AID。
