用于结核分枝杆菌的密码子优化的DsRed荧光蛋白。
Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis.
作者信息
Carroll Paul, Muwanguzi-Karugaba Julian, Parish Tanya
机构信息
Queen Mary University of London, Barts & The London School of Medicine and Dentistry, London, UK.
Infectious Disease Research Institute, Seattle, WA, 98102, USA.
出版信息
BMC Res Notes. 2018 Oct 1;11(1):685. doi: 10.1186/s13104-018-3798-3.
OBJECTIVE
We have previously codon-optimized a number of red fluorescent proteins for use in Mycobacterium tuberculosis (mCherry, tdTomato, Turbo-635). We aimed to expand this repertoire to include DsRed, another widely used and flexible red fluorescent protein.
RESULTS
We generated expression constructs with a full length DsRed under the control of one of three strong, constitutive promoters (P, P or P) for use in mycobacteria. We confirmed that full length DsRed (225 amino acids) was expressed and fluoresced brightly. In contrast to mCherry, truncated versions of DsRed lacking several amino acids at the N-terminus were not functional. Thus, we have expanded the repertoire of optimized fluorescent proteins for mycobacteria.
目的
我们之前对多种红色荧光蛋白进行了密码子优化，以用于结核分枝杆菌（mCherry、tdTomato、Turbo-635）。我们旨在扩大这个蛋白库，使其包括另一种广泛使用且灵活的红色荧光蛋白DsRed。
结果
我们构建了在三种强组成型启动子（P、P或P）之一控制下的全长DsRed表达构建体，用于分枝杆菌。我们证实全长DsRed（225个氨基酸）能够表达并发出明亮荧光。与mCherry不同，在N端缺少几个氨基酸的DsRed截短版本没有功能。因此，我们扩大了用于分枝杆菌的优化荧光蛋白库。
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