Faculty of Science and Technology, Free University of Bolzano, Piazza Università 5, 39100, Bolzano, Italy.
Faculty of Science and Technology, Free University of Bolzano, Piazza Università 5, 39100, Bolzano, Italy.
Chemosphere. 2019 Jan;214:563-578. doi: 10.1016/j.chemosphere.2018.09.127. Epub 2018 Sep 22.
The long-term use of Cu-containing fungicides contaminates vineyards soils, which can induce Cu toxicity and nutrient imbalances in several plant species. The aim of this work was to evaluate the effect of Cu toxicity on two grapevine rootstocks, Fercal and 196.17, and to elucidate if intercropping with oat can alleviate grapevine Cu toxicity. Plants were hydroponically-cultivated and treated with different Cu concentrations. At harvest the biomass accumulation, the SPAD index and the symplastic and apoplastic root and leaves ionome were measured to evaluate possible synergistic and/or antagonistic effects on other micro- and macronutrients. The root exudation analysis was correlated with genes expression (VvPEZ-like), whereas PCA analysis performed on the grapevine and oat ionome revealed that both mono- and intercropped 196.17 rootstock display a positive effect on Zn and Mn in the root tissues at high Cu concentrations. An increase of Zn and Mn in roots was also reported for the intercropped Fercal rootstock at high Cu concentrations while an antagonistic relation was reported for root Zn concentration in the monocropped Fercal rootstock. Our results showed that grapevine and oat compete for nutrient uptake and that this phenomenon can possibly alleviate grapevine Cu toxicity. However, Fercal rootstock is able to take advantage from oat, while 196.17 is disadvantaged by the intercropping system. Even though intercropping system seems to be a valuable tool to counteract grapevine Cu toxicity, the application of this agricultural practice has shown to be species dependent and should be evaluated for each rootstock.
长期使用含铜杀菌剂会污染葡萄园土壤，从而导致几种植物的铜毒性和营养失衡。本工作的目的是评估铜毒性对两种葡萄砧木 Fercal 和 196.17 的影响，并阐明与燕麦间作对葡萄铜毒性的缓解作用。采用水培法栽培植物，并施以不同浓度的 Cu。收获时，测量生物量积累、SPAD 指数以及质外体和共质体根和叶片的离子组，以评估对其他微量和大量营养素的可能协同和/或拮抗作用。根分泌物分析与基因表达（VvPEZ-like）相关，而对葡萄和燕麦离子组进行的 PCA 分析表明，高 Cu 浓度下，单作和间作的 196.17 砧木均对根组织中的 Zn 和 Mn 表现出积极影响。高 Cu 浓度下，间作的 Fercal 砧木的根 Zn 浓度也有所增加，而单作的 Fercal 砧木的根 Zn 浓度则表现出拮抗关系。我们的结果表明，葡萄和燕麦竞争养分吸收，而这种现象可能缓解葡萄的铜毒性。然而，Fercal 砧木能够从燕麦中受益，而 196.17 则因间作系统而处于不利地位。尽管间作系统似乎是对抗葡萄铜毒性的有效工具，但这种农业实践的应用表现出对物种的依赖性，应针对每种砧木进行评估。
