快照：RNA 结构探测技术。
SnapShot: RNA Structure Probing Technologies.
机构信息
Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca NY; Center for Synthetic Biology, Northwestern University, Evanston IL.
Department of Chemical and Biological Engineering, Northwestern University, Evanston IL.
出版信息
Cell. 2018 Oct 4;175(2):600-600.e1. doi: 10.1016/j.cell.2018.09.024.
Abstract
Chemical probing coupled to high-throughput sequencing offers a flexible approach to uncover many aspects of RNA structure relevant to its cellular function. With a wide variety of chemical probes available that each report on different features of RNA molecules, a broad toolkit exists for investigating in vivo and in vitro RNA structure and interactions with other molecules.
摘要
化学探针与高通量测序相结合，为揭示 RNA 结构与其细胞功能相关的多个方面提供了一种灵活的方法。有多种不同的化学探针可供选择，每种探针都能报告 RNA 分子的不同特征，因此存在一个广泛的工具包，可用于研究体内和体外 RNA 结构以及与其他分子的相互作用。
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