Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 210029, P,R, China.
BMC Genomics. 2014 Jan 18;15:42. doi: 10.1186/1471-2164-15-42.
Anopheles sinensis is an important mosquito vector of Plasmodium vivax, which is the most frequent and widely distributed cause of recurring malaria throughout Asia, and particularly in China, Korea, and Japan.
We performed 454 next-generation sequencing and obtained a draft sequence of A. sinensis assembled into scaffolds spanning 220.8 million base pairs. Analysis of this genome sequence, we observed expansion and contraction of several immune-related gene families in anopheline relative to culicine mosquito species. These differences suggest that species-specific immune responses to Plasmodium invasion underpin the biological differences in susceptibility to Plasmodium infection that characterize these two mosquito subfamilies.
The A. sinensis genome produced in this study, provides an important resource for analyzing the genetic basis of susceptibility and resistance of mosquitoes to Plasmodium parasites research which will ultimately facilitate the design of urgently needed interventions against this debilitating mosquito-borne disease.
中华按蚊是间日疟原虫的重要媒介蚊种，间日疟原虫是亚洲地区最常见和分布最广的复发性疟疾病原体，尤其在中国、韩国和日本。
我们进行了 454 次高通量测序，获得了一个组装成 2.208 亿碱基对的中华按蚊草图序列。对该基因组序列的分析表明，与库蚊种类相比，按蚊中几个与免疫相关的基因家族发生了扩张和收缩。这些差异表明，针对疟原虫入侵的种特异性免疫反应是这两个蚊亚科对疟原虫感染易感性存在生物学差异的基础。
本研究中产生的中华按蚊基因组为分析蚊子对疟原虫寄生虫易感性和抗性的遗传基础提供了重要资源，这将最终有助于设计对抗这种使人衰弱的蚊媒疾病的急需干预措施。
