Department of Dermatology, Venereology and Pediatric Dermatology, Medical University of Lublin, Lublin, Poland.
Department of Clinical Immunology and Immunotherapy, Medical University of Lublin, Chodzki 4a, 20-093, Lublin, Poland.
Arch Immunol Ther Exp (Warsz). 2019 Apr;67(2):103-108. doi: 10.1007/s00005-018-0527-5. Epub 2018 Oct 5.
Psoriasis is a chronic debilitating skin disease with an estimated prevalence reaching 2% of the worldwide population. Psoriatic disease is driven by a network of complicated reciprocal interactions among innate and adaptive mechanisms of immune system with structural components of the skin. Interleukin (IL)-22 mediates keratinocyte proliferation and epidermal hyperplasia, inhibits terminal differentiation of keratinocytes, and induces the production of antimicrobial proteins. The aim of this study was the assessment of IL-22 levels and its correlation with disease activity in plaque psoriasis. The study group included 64 patients with mild, moderate and severe psoriasis. Control group was composed of 24 sex- and age-matched healthy volunteers. IL-22 concentration was assessed in supernatants of T-cell cultures as well as in the plasma of study and control group with the use of ELISA method. Statistical analysis showed that concentration of IL-22 in cultures exposed to staphylococcal enterotoxin B was significantly higher than in control samples (p = 0.005) and cultures treated with IL-12 (p = 0.005). Patients with psoriasis presented significantly higher concentrations of IL-22 than healthy individuals (p = 0.0000001). In conclusion, IL-22 may collaborate with other soluble factors and cells together forming inflammatory circuits that otherwise exist as constitutive or inducible pathways in normal skin and become pathologically amplificated in psoriasis. Targeting IL-22 may be promising as a potential therapeutic for plaque psoriasis.
银屑病是一种慢性致残性皮肤病，估计全球患病率达到 2%。银屑病是由免疫系统的固有和适应性机制与皮肤的结构成分之间复杂的相互作用网络驱动的。白细胞介素 (IL)-22 介导角质形成细胞增殖和表皮增生，抑制角质形成细胞的终末分化，并诱导抗菌蛋白的产生。本研究旨在评估银屑病斑块中 IL-22 水平及其与疾病活动度的相关性。研究组包括 64 例轻度、中度和重度银屑病患者。对照组由 24 名性别和年龄匹配的健康志愿者组成。使用 ELISA 法评估 T 细胞培养物上清液和研究组及对照组血浆中 IL-22 的浓度。统计分析显示，暴露于葡萄球菌肠毒素 B 的培养物中 IL-22 的浓度明显高于对照样本（p=0.005）和 IL-12 处理的培养物（p=0.005）。银屑病患者的 IL-22 浓度明显高于健康个体（p=0.0000001）。总之，IL-22 可能与其他可溶性因子和细胞一起协同作用，共同形成炎症回路，否则这些回路在正常皮肤中作为组成性或诱导性途径存在，并在银屑病中病理性放大。靶向 IL-22 可能是治疗斑块状银屑病的一种有前途的方法。
