Shekarchi I C, Fuccillo D A, Strouse R, Sever J L
J Clin Microbiol. 1987 Feb;25(2):320-2. doi: 10.1128/jcm.25.2.320-322.1987.
Capillary enzyme immunosorbent assay (CapELISA) is a modification of the standard enzyme immunosorbent assay which permits rapid detection of viral antigens in clinical specimens. The capillary tube format provides a very large reactive surface relative to the sample size. The close proximity of antigen to antibody in the tube optimizes the reaction, resulting in increased sensitivity and shorter incubation requirements. Sensitivity is further enhanced by use of a biotin-avidin enzyme detector system and a fluorogenic rather than a colorigenic substrate. The assay is performed at ambient temperature and requires less than 2 h. It is read with an inexpensive hand-held black light. Data for the use of CapELISA for the detection of herpes simplex virus in clinical specimens are presented. The results show greater than or equal to 85% sensitivity and 100% specificity when compared with tissue culture tests on the same samples. This new system should be advantageous for the diagnosis, treatment, and management of patients with herpesvirus infections and for the prevention of neonatal herpes acquired by passage of the fetus through an infected birth canal.
毛细管酶免疫吸附测定法（CapELISA）是标准酶免疫吸附测定法的一种改进方法，可快速检测临床标本中的病毒抗原。相对于样本量而言，毛细管形式提供了非常大的反应表面。管内抗原与抗体的紧密接近优化了反应，从而提高了灵敏度并缩短了孵育时间。通过使用生物素-抗生物素蛋白酶检测系统和荧光底物而非显色底物，灵敏度进一步提高。该测定在室温下进行，耗时不到2小时。用廉价的手持式黑光读取结果。本文展示了使用CapELISA检测临床标本中单纯疱疹病毒的数据。与对相同样本进行的组织培养测试相比，结果显示敏感性大于或等于85%，特异性为100%。这种新系统对于疱疹病毒感染患者的诊断、治疗和管理以及预防胎儿通过受感染产道获得的新生儿疱疹应该具有优势。
