Halbert S P, Anken M
J Infect Dis. 1977 Oct;136 Suppl:S318-23. doi: 10.1093/infdis/136.supplement_2.s318.
An enzyme-labeled "sandwich" assay was developed for the detection of the hepatitis B surface antigen (HBS Ag) with use of calf intestinal alkaline phosphatase and horse antibody to HBS Ag. It involved a total incubation time of 2.5 hr in three steps and was approximately equivalent in sensitivity to one of the commercially available radioimunoassays. The confirmatory test was of the neutralization type and required an additional 30-min incubation step. The enzyme-labeled antibody solution and the solid-phase antibody were extremely stable. They withstood storage at 2 C-8 C for longer than one year with virtually no decrease in activity. Accelerated tests of stability in storage at room temperature (20 C-24 C) showed only a small loss of potency over a one-year period. Three specimens were encountered that were confirmably positive by enzyme-labeled immunoassay but negative by radioimmunoassay.
利用小牛肠碱性磷酸酶和抗乙肝表面抗原（HBS Ag）的马抗体，开发了一种酶标记“夹心”检测法用于检测乙肝表面抗原（HBS Ag）。该检测法分三步进行，总孵育时间为2.5小时，灵敏度与市售放射免疫分析法之一大致相当。确证试验为中和型，需要额外30分钟的孵育步骤。酶标记抗体溶液和固相抗体极其稳定。它们在2℃至8℃下储存一年以上，活性几乎没有下降。在室温（20℃至24℃）下储存的加速稳定性试验表明，在一年时间里效力仅略有损失。遇到了三个标本，经酶标记免疫分析确证为阳性，但经放射免疫分析为阴性。
