使用临床标本对杜邦HERPCHEK单纯疱疹病毒抗原检测进行评估。
Evaluation of the Du Pont HERPCHEK herpes simplex virus antigen test with clinical specimens.
作者信息
Wu T C, Zaza S, Callaway J
机构信息
Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
J Clin Microbiol. 1989 Aug;27(8):1903-5. doi: 10.1128/jcm.27.8.1903-1905.1989.
The HERPCHEK herpes simplex virus (HSV) antigen test (Du Pont, Billerica, Mass.) is a biotinstreptavidin-amplified enzyme-linked immunoassay for the detection of HSV antigen directly in clinical specimens. In a total of 200 mucocutaneous specimens, predominantly lesions from nongenital sites, HERPCHEK had a sensitivity of 90% and a specificity of 99% compared with the shell vial tissue culture method which uses primary rabbit kidney cells. Five false-negative results by HERPCHEK were found with specimens that contained a low percentage of positive cells by the direct immunoperoxidase stain used in a tissue culture method (1 to 9%). The absorbance values of the clinical specimens by the HERPCHEK test do not correlate well with the percentage of positive cells. Our results indicate that the HERPCHEK HSV antigen test, when performed with specimens from lesions, is a sensitive and specific test as compared with shell vial culture.
HERPCHEK单纯疱疹病毒（HSV）抗原检测（杜邦公司，马萨诸塞州比勒里卡）是一种生物素-链霉亲和素放大酶联免疫测定法，用于直接在临床标本中检测HSV抗原。在总共200份黏膜皮肤标本中，主要是来自非生殖器部位的病变，与使用原代兔肾细胞的空斑小瓶组织培养法相比，HERPCHEK的灵敏度为90%，特异性为99%。通过HERPCHEK检测发现有5例假阴性结果，这些标本在组织培养法中使用的直接免疫过氧化物酶染色显示阳性细胞百分比很低（1%至9%）。HERPCHEK检测的临床标本吸光度值与阳性细胞百分比相关性不佳。我们的结果表明，与空斑小瓶培养相比，用HERPCHEK HSV抗原检测法检测病变标本时，该检测法灵敏且特异。
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