miR-183-5p 通过调控 RGS2 促进乳腺癌发生发展。
miR-183-5p Aggravates Breast Cancer Development via Mediation of RGS2.
机构信息
Department of Breast Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China.
Department of Breast Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China.
出版信息
Comput Math Methods Med. 2021 Nov 20;2021:9664195. doi: 10.1155/2021/9664195. eCollection 2021.
This study mainly explores how miR-183-5p pertains to breast cancer (BC) development. Functional assays were employed to test impacts of miR-183-5p in this cancer. Targeting between RGS2 and miR-183-5p was examined with dual-luciferase assay, and how their interaction pertains to cancer progression was further unraveled. miR-183-5p level was noticeably high in cancer tissue/cells. Overexpressing miR-183-5p could remarkably deteriorate cancer progression. The regulatory gene RGS2 levels was markedly low in BC, and two genes we researched were negatively correlated. It was uncovered by rescue assay that miR-183-5p/RGS2 axis mediated tumor-relevant behaviors in BC. Altogether, miR-183-5p aggravates BC development via mediation of RGS2. miR-183-5p supplies a promising target for BC therapy.
本研究主要探讨 miR-183-5p 与乳腺癌 (BC) 发展的关系。采用功能分析检测 miR-183-5p 在这种癌症中的影响。通过双荧光素酶报告基因实验检测 RGS2 和 miR-183-5p 之间的靶向关系，并进一步阐明它们的相互作用与癌症进展的关系。miR-183-5p 在肿瘤组织/细胞中明显高表达。过表达 miR-183-5p 可显著恶化癌症进展。RGS2 基因在 BC 中表达明显下调，我们研究的两个基因呈负相关。通过挽救实验发现，miR-183-5p/RGS2 轴介导了 BC 中的肿瘤相关行为。综上所述，miR-183-5p 通过调节 RGS2 加剧了 BC 的发展。miR-183-5p 为 BC 治疗提供了一个有前途的靶点。
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