Khan Nasima, Afroz Farhana, Begum Mst Nadira, Roy Rony Satyajit, Sharmin Suriya, Moni Fatema, Mahmood Hasan Choudhury, Shaha Koushik, Sohrab Md Hossain
Department of Chemistry, Jahangirnagar University, Savar, Dhaka, 1342, Bangladesh.
Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dhaka, 1205, Bangladesh.
Toxicol Rep. 2018 Sep 3;5:970-976. doi: 10.1016/j.toxrep.2018.08.016. eCollection 2018.
This study reports the chemical investigation and bioactivity of the secondary metabolites produced by the endophytic fungus isolated from Linn. growing in Bangladesh. This plant was collected from conservation forest in Bangladesh and belongs to the Caesalpiniaceae family. The endophytic fungus was isolated from the tissue of root of this plant. The fungal strain was identified by morphological characters and DNA sequencing. The crude organic extract of the fungal strain was proven to be active when tested for cytotoxicity against Brine Shrimp Lethality Bioassay, antimicrobial and antioxidant activity. The bioactivity guided fractionation of the ethyl acetate extract leads to the isolation of seven secondary metabolites in pure form. The structures of the isolated compounds were determined by the analysis of NMR and mass spectroscopic data. Bioassay investigation of the isolated secondary metabolites suggested aza-anthraquinones are more potent bioactive compounds as anticancer and antimicrobial agent.
本研究报道了从生长在孟加拉国的 Linn. 中分离出的内生真菌产生的次生代谢产物的化学研究及生物活性。该植物采自孟加拉国的保护森林，属于苏木科。内生真菌从该植物的根部组织中分离得到。通过形态特征和 DNA 测序对真菌菌株进行了鉴定。经卤虫致死生物测定法测试其细胞毒性、抗菌和抗氧化活性，结果表明该真菌菌株的粗有机提取物具有活性。乙酸乙酯提取物的生物活性导向分级分离导致以纯形式分离出七种次生代谢产物。通过对核磁共振和质谱数据的分析确定了分离出的化合物的结构。对分离出的次生代谢产物的生物测定研究表明，氮杂蒽醌作为抗癌和抗菌剂是更有效的生物活性化合物。
