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Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame.

作者信息

Grönger P, Manian S S, Reiländer H, O'Connell M, Priefer U B, Pühler A

出版信息

Nucleic Acids Res. 1987 Jan 12;15(1):31-49. doi: 10.1093/nar/15.1.31.

DOI:10.1093/nar/15.1.31
PMID:3029674
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340396/
Abstract

By hybridization and heteroduplex studies the fixABC and nifA genes of the Rhizobium leguminosarum symbiotic plasmid pRL6JI have been identified. DNA sequencing of the region containing nifA showed an open reading frame of 1557 bp encoding a protein of 56, 178 D. Based on sequence homology, this ORF was confirmed to correspond to the nifA gene. Comparison of three nifA proteins (Klebsiella pneumoniae, Rhizobium meliloti, Rhizobium leguminosarum) revealed only a weak relationship in their N-terminal regions, whereas the C-terminal parts exhibited strong homology. Sequence analysis also showed that the R. leguminosarum nifA gene is followed by nifB and preceded by fixC with an open reading frame inserted in between. This novel ORF of 294 bp was found to be highly conserved also in R. meliloti. No known promoter and termination signals could be defined on the sequenced R. leguminosarum fragment.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e581/340396/a59772dae6b6/nar00245-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e581/340396/a59772dae6b6/nar00245-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e581/340396/a59772dae6b6/nar00245-0050-a.jpg

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本文引用的文献

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Organization and expression of Rhizobium meliloti nitrogen fixation genes.根瘤菌 meliloti 氮固定基因的组织和表达。
Proc Natl Acad Sci U S A. 1983 May;80(10):3005-9. doi: 10.1073/pnas.80.10.3005.
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Mapping and expression of a regulatory nitrogen fixation gene (fixD) of Rhizobium meliloti.苜蓿中华根瘤菌调控固氮基因（fixD）的定位与表达。
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Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA-like gene.
苜蓿中华根瘤菌 nifA 基因的表达与功能分析。
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Transcriptional analysis of biofilm formation processes in the anaerobic, hyperthermophilic bacterium Thermotoga maritima.嗜热栖热菌厌氧嗜热生物膜形成过程的转录分析
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The fixABCX genes in Rhodospirillum rubrum encode a putative membrane complex participating in electron transfer to nitrogenase.深红红螺菌中的fixABCX基因编码一种推定的膜复合物，参与向固氮酶的电子传递。
J Bacteriol. 2004 Apr;186(7):2052-60. doi: 10.1128/JB.186.7.2052-2060.2004.
7
Bradyrhizobium japonicum possesses two discrete sets of electron transfer flavoprotein genes: fixA, fixB and etfS, etfL.慢生根瘤菌拥有两组不同的电子传递黄素蛋白基因：fixA、fixB和etfS、etfL。
Arch Microbiol. 1996 Mar;165(3):169-78. doi: 10.1007/BF01692858.
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Arch Microbiol. 1995 Aug;164(2):142-51. doi: 10.1007/BF02525320.
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Analysis of regulation of Klebsiella pneumoniae nitrogen fixation (nif) gene cluster with gene fusions.利用基因融合技术对肺炎克雷伯菌固氮（nif）基因簇调控的分析。
Nature. 1980 Jul 10;286(5769):128-32. doi: 10.1038/286128a0.
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