运动干预改变肥胖女性 HDL 亚类分布和功能。

Exercise intervention alters HDL subclass distribution and function in obese women.

机构信息

Hatter Institute for Cardiovascular Research in Africa, Department of Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.

Non-Communicable Diseases Research Unit, South African Medical Research Council, Cape Town, South Africa.

出版信息

Lipids Health Dis. 2018 Oct 10;17(1):232. doi: 10.1186/s12944-018-0879-1.

BACKGROUND

Obesity is associated with a change in high-density lipoprotein (HDL) function and subclass. Exercise training reduces cardiovascular risk in obese patients. We aimed to explore the effect of an exercise training stimulus on HDL functionality and subclass in obese women.

METHODS

Thirty-two obese black South African women were randomly assigned to exercise (combined aerobic and resistance exercise) or control (no exercise) conditions for 12-weeks. Pre- and post-testing included venous blood sampling for analysis of lipid profile and HDL functionality, by measuring cellular cholesterol efflux capacity, reduction in endothelial vascular cell adhesion molecule (VCAM) expression (anti-inflammatory function), paraoxonase (PON) (antioxidative function) and platelet activating factor acetylhydrolase (PAF-AH) activities (anti-thrombotic function). PON-1 and PAF-AH expression were determined in serum and in isolated HDL using Western blotting. Levels of large, intermediate and small HDL subclasses were measured using the Lipoprint® system.

RESULTS

Exercise training resulted in a decrease in body mass index (- 1.0 ± 0.5% vs + 1.2 ± 0.6%, p = 0.010), PON activity (- 8.7 ± 2.4% vs + 1.1 ± 3.0%, p = 0.021), PAF-AH serum expression (- 22.1 ± 8.0% vs + 16.9 ± 9.8, p = 0.002), and the distribution of small HDL subclasses (- 10.1 ± 5.4% vs + 15.7 ± 6.6%, p = 0.004) compared to controls. Exercise did not alter HDL cellular cholesterol efflux capacity and anti-inflammatory function.

CONCLUSIONS

These results demonstrate the potential for exercise training to modify HDL subclass distribution and HDL function in obese women.

TRIAL REGISTRATION

Clinical trials number: PACTR201711002789113 .

背景

肥胖与高密度脂蛋白（HDL）功能和亚类的改变有关。运动训练可降低肥胖患者的心血管风险。我们旨在探讨运动训练刺激对肥胖女性 HDL 功能和亚类的影响。

方法

32 名肥胖的南非黑人女性被随机分配到运动（有氧和阻力运动相结合）或对照组（不运动）条件下进行 12 周的治疗。在治疗前后，通过测量细胞胆固醇流出能力、内皮血管细胞黏附分子（VCAM）表达减少（抗炎功能）、对氧磷酶（PON）（抗氧化功能）和血小板激活因子乙酰水解酶（PAF-AH）活性（抗血栓形成功能），采集静脉血样分析血脂谱和 HDL 功能。使用 Western blot 法在血清和分离的 HDL 中测定 PON-1 和 PAF-AH 的表达。使用 Lipoprint®系统测量大、中、小 HDL 亚类的水平。

结果

运动训练导致体重指数下降（-1.0±0.5% vs +1.2±0.6%，p=0.010）、PON 活性下降（-8.7±2.4% vs +1.1±3.0%，p=0.021）、PAF-AH 血清表达下降（-22.1±8.0% vs +16.9±9.8%，p=0.002）和小 HDL 亚类分布减少（-10.1±5.4% vs +15.7±6.6%，p=0.004），与对照组相比。运动并未改变 HDL 细胞胆固醇流出能力和抗炎功能。