a Department of Oral Biochemistry , Chonbuk National University , Jeonju , Korea.
b Department of Dental Materials , Chonbuk National University , Jeonju , Korea.
Artif Cells Nanomed Biotechnol. 2018;46(sup3):S807-S817. doi: 10.1080/21691401.2018.1513940. Epub 2018 Oct 11.
Osseointegration of dental implants is affected by osteoporosis. The purpose of this study was overcome the implant failure and facilitate the osseointegration of dental implants by c-myb in ovariectomized (OVX)-induced osteoporosis. c-myb is a transcription factor and supports bone formation. Plasmid DNA/c-myb conjugated with chitosan-gold nanoparticles (Ch-GNPs/c-myb) promoted osteogenesis and inhibited osteoclastogenesis in MC-3T3 E1 cells. Ch-GNPs/c-myb involved the reduction of the nuclear factor of activated T-cells 1, c-Fos, and tartrate-resistant acid phosphatase-positive multinucleated osteoclasts in receptor activator of nuclear factor-κB ligand (RANKL) stimulated bone marrow macrophages. In vivo results of rat mandibles demonstrated Ch-GNP/c-myb-coated titanium (Ti) implants increased the volume and density of newly formed bone and the osseointegration of dental implant with bone by micro computed tomography examination after OVX-induced osteoporosis. Immunohistochemical analysis showed increased c-myb expression and upregulation of bone morphogenic proteins, osteoprotegerin and EphB4, as well as the downregulation of RANKL by Ch-GNP/c-myb-coated Ti implants. Hematoxylin and Eosin staining expressed new bone formation by Ch-GNP/c-myb-coated Ti implants. Our findings indicated that c-myb delivered by Ch-GNPs supports osseointegration of dental implant even in osteoporotic condition. c-myb may be applicable to support dental implant integration and treatment in age-dependent bone destruction disease.
牙种植体的骨整合受骨质疏松症的影响。本研究的目的是通过 c-myb 克服种植体失败并促进去卵巢（OVX）诱导的骨质疏松症中的牙种植体的骨整合。c-myb 是一种转录因子，支持骨形成。与壳聚糖-金纳米粒子（Ch-GNPs/c-myb）偶联的质粒 DNA/c-myb 促进了 MC-3T3 E1 细胞中的成骨作用并抑制了破骨细胞形成。Ch-GNPs/c-myb 涉及核因子活化 T 细胞 1、c-Fos 和抗酒石酸酸性磷酸酶阳性多核破骨细胞的减少，在核因子-κB 配体（RANKL）刺激的骨髓巨噬细胞中。大鼠下颌骨的体内结果表明，Ch-GNP/c-myb 涂层钛（Ti）植入物增加了体积和新形成骨的密度，以及 OVX 诱导的骨质疏松症后微计算机断层扫描检查的牙种植体的骨整合。免疫组织化学分析显示，Ch-GNP/c-myb 涂层 Ti 植入物增加了 c-myb 的表达和骨形态发生蛋白、骨保护素和 EphB4 的上调，以及 RANKL 的下调。Ch-GNP/c-myb 涂层 Ti 植入物通过苏木精和伊红染色表达了新骨形成。我们的研究结果表明，Ch-GNPs 递送的 c-myb 甚至在骨质疏松症情况下也支持牙种植体的骨整合。c-myb 可适用于支持与年龄相关的骨破坏疾病中的牙种植体整合和治疗。
